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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 771 results for: Vars;

Showing: 41 - 60 of 771 results

  1. Immunocytochemistry

    Type
    Protocol
    ...will vary depending on the sample type and the protein of interest. You may need to try a variety of fixation... Pro-Tip The optimal antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL... target. The optimal antibody concentration will vary between antibodies. Review the manufacturer's instructions...

  2. Brain Initiative Collection

    Type
    Collection
    ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom...

  3. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ... activating or upregulating gene expression in various model organisms....specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

  4. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

  5. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression...

  6. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting...

  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. gRNAs gRNA plasmids to target specific...

  8. Retrovirus Plasmids

    Type
    Collection
    ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory...

  9. Antibody Production

    Type
    Collection
    ...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

  10. mTOR Pathway

    Type
    Collection
    ...mTORC2 The better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen,...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

  11. Promega Plasmid Collection

    Type
    Collection
    ...which have been developed for special functions that vary from purification, visualization, and functional...specific purpose. HaloTag technology is optimized for various protein expression systems including E. coli , ...

  12. AAV Packaged on Request

    Type
    Collection
    ...standards and typical titers, though actual yields may vary by construct. Size Quantity Total Volume Minimum...processing your order. MTA Approval 1+ business days, varies by requesting organization Viral vector preps remain...

  13. p53 Pathway

    Type
    Collection
    ...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20. PubMed PMID...

  14. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...express your gene of interest under the control of a variety of promoters. Professor Oka and the Baylor College...can be used to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases...

  16. Lentivirus Production

    Type
    Protocol
    ...reagents. Once produced, lentivirus can be used for a variety of downstream applications such as stable-cell ...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ... cm dish. Pro-Tip There can be batch to batch variation when making the PEI working stock, therefore the...transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1-2 days after transfection...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination events but...

  19. DNA Quantification

    Type
    Protocol
    ...Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength that...spectrophotometer between samples if they are expected to vary significantly in concentration. DNA dissolved in...in water is going to have a greater variance in concentration readings than a DNA sample dissolved in ...

  20. Fluorescence Titering Assay

    Type
    Protocol
    ...developed for 293T cells but can be adapted to a variety of target cell lines and selection markers. Note...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...viability and transduction efficiency. Titer will vary between cell lines. It is not recommended to subject...
Showing: 41 - 60 of 771 results