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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 775 results for: Vars;

Showing: 41 - 60 of 775 results

  1. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ... activating or upregulating gene expression in various model organisms....specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

  2. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

  3. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting...

  4. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. gRNAs gRNA plasmids to target specific...

  6. Retrovirus Plasmids

    Type
    Collection
    ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory...

  7. Antibody Production

    Type
    Collection
    ...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

  8. mTOR Pathway

    Type
    Collection
    ...mTORC2 The better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen,...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

  9. Promega Plasmid Collection

    Type
    Collection
    ...which have been developed for special functions that vary from purification, visualization, and functional...specific purpose. HaloTag technology is optimized for various protein expression systems including E. coli , ...

  10. AAV Packaged on Request

    Type
    Collection
    ...standards and typical titers, though actual yields may vary by construct. Size Quantity Total Volume Minimum...processing your order. MTA Approval 1+ business days, varies by requesting organization Viral vector preps remain...

  11. Lentivirus Production

    Type
    Protocol
    ...reagents. Once produced, lentivirus can be used for a variety of downstream applications such as stable-cell ...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ... cm dish. Pro-Tip There can be batch to batch variation when making the PEI working stock, therefore the...transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1-2 days after transfection...

  12. p53 Pathway

    Type
    Collection
    ...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20. PubMed PMID...

  13. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...express your gene of interest under the control of a variety of promoters. Professor Oka and the Gene Vector...can be used to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases...

  15. COVID-19 Resources

    Type
    Collection
    ...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV...Screening Jesse Bloom Tyler Starr Libraries of variant Spike protein RBD mutations for comprehensive mapping...

  17. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination events but...

  18. DNA Quantification

    Type
    Protocol
    ...Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength that...spectrophotometer between samples if they are expected to vary significantly in concentration. DNA dissolved in...in water is going to have a greater variance in concentration readings than a DNA sample dissolved in ...

  19. Fluorescence Titering Assay

    Type
    Protocol
    ...developed for 293T cells but can be adapted to a variety of target cell lines and selection markers. Note...FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one suitable ...viability and transduction efficiency. Titer will vary between cell lines. It is not recommended to subject...

  20. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... enzymes, allowing scientists to target a wide variety of recognition sequences. For a list of many commonly... 1 µg of DNA. The total reaction volume usually varies from 10-50 µL depending on application and is largely...is due to "Star Activity" and can happen for a variety of reasons, including high glycerol concentration...
Showing: 41 - 60 of 775 results