We narrowed to 702 results for: abo.1

...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development... the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...

...and interrogate with conventional reagents. Figure 1: Axon guidance cues and receptors regulate the development...of cell surface and secreted proteins from a collaboration between Addgene and IPI. Antibodies...antibodies in an open science model. Learn more about IPI (Link opens in a new window) Axon Guidance IPI...

...1774 for neomycin selection. Bob Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...Gamma-retroviral Vector Guide for more information about gamma-retroviral components, viral production, and...

... Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. Your ...tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2...systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in ...AAV Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your cell type...

...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for...quality control partner before distribution. Details about our production protocols, titering methods, and ...Depositing Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for...

... your account. We will notify you by email within 1 business day regarding your account verification. ...plasmids that are available to industry. Learn more about the available plasmids and how to order them. Distribution...Information page for more information. To learn more about Addgene's MTA process please visit our Help Center...

...cell surface proteins. J Am Chem Soc. 127(37):12770-1. PMID:16159249...available Non-toxic to living cells To learn more about this system, visit the New England Biolabs website...

...integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids...optimized for both safety and efficiency. Learn more about the different types of viral vectors and find viral...

...available as a substrate for another round. Figure 1: Overview of prime editing. Created with BioRender.com...for CRISPR prime editing plamisds. To learn more about prime editing and other CRISPR topics, read our ...

...coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease...researchers who are learning to wield them. Learn more about Addgene’s TAL effector and TALEN kits here . References...

...serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid, expressing Rep2 and Cap1... Read our AAV vector guide for more information about AAV components, generations, viral production, and...

...to determine purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV ... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...

...2017. 1. Among scientists, historians have the most Twitter users. Biologists rank #8. Figure 1: Highly...that these results could be biased for two reasons: 1) they are based on the US Occupational Employment ...female to male = 0.629 (the closer this ratio is to 1, the closer males and females are to parity - the ... authorship in the United States (0.428). 4. The #1 (re)tweeted domain by scientists was Instagram Social...links to research that isn’t behind paywalls. 5. The #1 (re)tweeted science domain by scientists was Nature...use Twitter professionally to network, learn more about alternative careers for scientists, and share cool....” This paper addresses the following questions about scientists on Twitter: who are they? What do they...

...one of three different inherited genes: Presenilin 1, Presenilin 2, and APP. The majority (> 90%) of individuals...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

..., 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR tips...Developed by the Fredrik Wermeling Lab . Read more about it on the Addgene blog . CRISPOR (Link opens in ... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...maintained by the Jennifer Doudna Lab . Read more about it on the Addgene blog . CRISPRflydesign (Link opens...

...and placed in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made... together to form a “Y” shape, as shown in Figure 1. The two arms of the Y structure are responsible for...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...

...cells . Signal transduction and targeted therapy, 5 (1), 283. https://doi.org/10.1038/s41392-020-00426-x ...lightning speed to elucidate as much as they could about this novel virus. SARS-CoV-2 entry ...

...plasma HIV-1, improving life expectancy and quality for HIV-1 patients. However, ART is not an HIV-1 cure. ...activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic...with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number...currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies ...HIV-1 transcription and destroy infected cells, the other using wild-type Cas9 to remove the HIV-1 genome...take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and HIV-1 reservoirs...Cutting HIV-1 out of the genome with CRISPR/Cas9 Before the introduction of CRISPR, HIV-1 genome editing...

...Control Viviana Gradinaru 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell...serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced...Janelia Research Campus viral project team in collaboration with David Schaffer (University of California...

...  Figure 1: Virtual lab meeting? No problem! You can still creatively collaborate and brainstorm... on it – so it is important to take breaks. After 1 hour (or another designated time) of meeting, someone...else to focus on. Use collaborative cloud apps It is a good idea to use collaborative cloud tools such as...creative collaboration in the workplace. There are many other ways to encourage collaborations remotely...serve as a springboard for future discussion. Collaboration and creativity are pivotal in writing publications...Vicens et al., 2007) geared towards enhancing collaborative writing and this recent article published in...approaches/tools we have to foster creative collaboration remotely in science are endless. Let’s adapt...