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Showing: 41 - 60 of 625 results

  1. TALEN Guide

    Type
    Collection
    ...studies suggest the likelihood of context having a negative effect on specificity is on par with ZF arrays...Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit. In this example, the final array is composed...that can be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments... Nature Biotechnology in August, uses a serial ligation protocol to assemble arrays. Dr. Joung, co-founder...to mutate a specific gene or your anxiously investigating gene therapy options for a rare disease in humans...

  2. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IRSp53/BAIAP2 Human Mouse IgG2a 177463 Anti-VGAT [L118/80R] VGAT Mouse Mouse IgG2a 177464 Anti-NPY2R [L119...receptor, type 1 Rat Mouse IgG2a 188172 Anti-VGAT [L118/14R] VGAT Mouse Mouse IgG2a 188173 Anti-NPY2R/Neuropeptide... K+ channel Rat Mouse IgG2a 188176 Anti-VGAT [L118/135R] VGAT Mouse Mouse IgG2a 188177 Anti-IP3 receptor...Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT [L118/80R-2b] VGAT Mouse Mouse IgG2b 206641 Anti-CALB2/calretinin...] Homer1L Mouse Mouse IgG2a 206689 Anti-VGAT [L118/80R-1] VGAT Mouse Mouse IgG2a 206690 Anti-CALB2/calretinin...) rat IgG2a 227081 VGAT (Mus musculus) recombinant mouse monoclonal antibody. VGAT Mouse Chimera: M. musculus...Kv1.7 K+ channel Mouse Mouse 206754 VGAT scFv [L118/14] L118/14 scFv VGAT Mouse Mouse 206755 IP3 receptor,...

  3. Lentivirus Plasmids

    Type
    Collection
    ... plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible... Tet-based inducible shRNA or cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene...of pSLIK. Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos...PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for CMV driven expression of cDNA. See ...pCW57.1 3rd Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions...

  4. Neurodegeneration Research Collection

    Type
    Collection
    ...treating them. Scientists are studying protein aggregation, learning about the blood brain barrier integrity...glutamine-rich sequences are prone to misfolding and aggregation and can interfere with protein-protein interactions...other large scale studies. Researchers are now investigating the role that these additional genes may play... and Noteworthy: Use tau constructs to study aggregation. Saha et al. Nat Commun. 2023 Feb 2. Study the...Induce CNS cells with concomitant expression of aggregation-prone proteins with the iPSC toolbox . Lam et...

  5. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy...complement oligo (100 μM; see step 1.4 ), 1.0 μl 10x T4 Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide...These cycling conditions allow for digestion and ligation to occur in one reaction (see step 3.2 ). Transform...using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC. This is a representative sequencing...

  6. Microbiology Resources

    Type
    Collection
    ... - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to ...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...which can result in unintended system induction. Investigate whether or not your serum is tetracycline-free...Lentiviral vector for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA...Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for conditional cell line generation... Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving tTA; contains...

  8. CRISPR References and Information

    Type
    Collection
    ...; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148 , pAC149 ; gateway destination: pAC90...MAGeCK). This tool can identify positively or negatively selected sgRNAs, genes, or pathways. CrispyCrunch...recombination Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed...to SAM Library AAV In vivo Plasmids In vivo interrogation of gene function in the mammalian brain using...

  10. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander et al., Nat Biotechnol...domain and flanked by restriction sites used for ligation into longer arrays. These 28 plasmids are available...

  11. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing... provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence... of the backbone with BbsI followed by a quick ligation of annealed and phosphorylated oligonucleotides...

  12. Resolute Plasmid Collection

    Type
    Collection
    ...RESOLUTE Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors...and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert...

  13. Antibody Production

    Type
    Collection
    ...for use in live cells and will interfere with conjugation. For applications such as these, the buffer can...species-specific horseradish peroxidase (HRP)-conjugated secondary antibody. The tissue labeling pattern... with the antibody and a species-specific HRP-conjugated secondary antibody. Protein expression is compared...

  14. Distribution to Industry

    Type
    Collection
    ...end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden Gate assembly... name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for...

  15. Qi Lab CRISPR Page

    Type
    Collection
    ...repression) by interfering with transcriptional elongation, RNA polymerase binding, or transcription factor...expression cassette, useful for selection or fluorescent gating of transiently transfected cells. Co-expression...promoter and sgRNA targeting GAL4 UAS promoter (negative control) 46918 pU6-sgCD71-2 Human pSico-based ...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...Morsch Lab. A vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly...Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino...HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders ...

  18. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...

  19. Chemogenetics Plasmids

    Type
    Collection
    ...popular in neuroscience, where they can be used to interrogate brain function and examine the relationship between...coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating or inhibitory...

  20. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication...Publication Additional Resources Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast...
Showing: 41 - 60 of 625 results