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Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Learn about CRISPR plant base editors for plants

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common uses.

Learn about using Optogenetics plasmids in your lab, including microbial opsins and optical switch systems.

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

Best practices for performing sequence analysis of a plasmid

Learn about hot new tools in our collection!

Learn more about NanoBiT technology and explore examples of HiBiT-tagged fusion proteins in action.

Learn how repressible promoters work and how you can use them in your next experiment.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization studies or organelle labeling.

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Learn what non-model organisms are, why scientists use them, and what they can teach us.