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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 677 results for: RON

Showing: 31 - 40 of 677 results

  1. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... 2 : Provides an optimal and stable chemical environment for the DNA polymerase to work adequately. Divalent...solvent and buffer capabilities provide a suitable environment for the DNA amplification reaction. Reference...

  2. Pouring LB Agar Plates

    Type
    Protocol
    ... strain grows, it's possible that: You used the wrong antibiotic. Your antibiotic is at too high a concentration... strain grows, it’s likely that you’ve used the wrong antibiotic, or confused your strains....

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...redox. J Cell Biol. 2013 Apr 15;201(2):337-49. David Ron Redox/ROS Thiol redox-sensitive ratiometric sensor...Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine...electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron. 2012 Sep 6;75(5):779-...and cellular processes, or other factors like environmental conditions. With proper experimental design,... fluorescence properties can be affected by environmental factors and the sensor behavior may differ from...High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods...Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ...and 30 bp annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins...

  8. Pipetting Protocol

    Type
    Protocol
    ...Background Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger ...
Showing: 31 - 40 of 677 results