We narrowed to 154 results for: 11
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TypeCollection...using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PubMed (Link opens in a new window) Chuhma...
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Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-4. (Link opens in a new window) Last reviewed... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post... of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter of RNA targets in a 40-minute incubation... -
CRISPR-mediated Plant Base Editors
TypeBlog Post..." Nature plants (2018): 1. PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice ... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...PMID: 28533371. PubMed Central PMCID: PMC5468623. 11. Várnai, Péter, and Tamas Balla. "Live cell imaging... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post... GTPase Signaling Toolbox. ACS Synthetic Biology, 11(1), 515–521. https://doi.org/10.1021/acssynbio.1c00604... -
Plasmids 101: Biotinylation
TypeBlog Post...PubMed PMID:12802011. PubMed Central PMCID: PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C... -
CRISPR History and Development for Genome Engineering
TypeCollection...classification of CRISPR-Cas systems. Nat Rev Microbiol . 13(11):722-36. PMID: 26411297 Mali P, Yang L, Esvelt KM... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post... Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503 Chamberlain... -
Hot Plasmids: Spring 2025
TypeBlog Post...insertions up to 12 kb — with efficiencies between 5% and 11%. It has higher efficiency and lower indel formation... -
Hot Plasmids: Summer 2024
TypeBlog Post...Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single alpha helix)... -
AAV Production in HEK293 Cells
TypeProtocol...55 mL of heat-inactivated FBS and 5 mL of glutaGRO 11 mL of 200 mM L-alanyl-L-glutamine. Store at 4 °C.... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...Students. Social Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van Duyne, Gregory D. ... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https://doi.org/10.1007/s11064... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000, doi:10.1073/pnas.100127597... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...biotechnology 29.9 (2011): 816-823. PubMed PMID: 21822255. 11. Wang, Xiaoling, et al. "Unbiased detection of off-target... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...PMID: 21527931. PubMed Central PMCID: PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...Stains and fluorescent dyes. Nature Cell Biology, 11(S1), S7–S7. https://doi.org/10.1038/ncb1939 Liu, ... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...pages using the links at the top of the page. Figure 11: A completed plasmid page for review before submission... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...This method resulted in efficiencies ranging from 11-100% of F1 animals carrying precise knock-ins, and... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...PMID: 23667515. PubMed Central PMCID: PMC3647052. 11. Stryer, Lubert. "Fluorescence energy transfer as... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/s41587-021-00938... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post... an ever increasing marketplace. Since 2013 about 11 tools have been added every year. Breaking down... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...using the CRISPR-Cas9 system. Nature Protocols, 8(11), 2281–2308. https://doi.org/10.1038/nprot.2013.143... -
CRISPR Guide
TypeGuide...orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria meningitidis ...orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview of visualizing genomic loci with fluorophores...optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927. PMID: 30377362 Casini, A., Olivieri, M...CRISPR–Cas systems. Nature Reviews Microbiology , 13 (11), 722–736. PMID: 26411297 Marino, N. D., Pinilla-...using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A., Cong, L.,...double-stranded breaks. Biochemical Journal . 475 (11), 1955–1964. PMID: 29891532 Gaudelli, N. M., Komor...genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID: 30403660 Sanson, K. R., Hanna, ... -
Chemogenetics Guide
TypeGuide...receptors couple to: Gq-DREADDs signal through the Gαq/11 G-protein and activate neuronal firing through stimulating...by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 (Link opens in a new window...with improved coupling efficiencies. Neurophotonics. 11:024208. PMID: 38559366 (Link opens in a new window... -
Gamma-Retroviral Vector Guide
TypeGuide...Vectors The genome of gamma-retroviruses ranges from 9–11 kb, encoded on RNA (Figure 1). This RNA is reverse...novel profiling tool in cancer genomics. PLoS ONE , 11 (4), e0154070. https://doi.org/10.1371/journal.pone... -
Optogenetics Guide
TypeGuide...excitation of distinct neural populations. Nat Methods. 11(3):338-46. PMID 24509633 Lin, J. 2011. A User’s Guide...control derived from Volvox carteri. Nat Neurosci. 11(6):631-3. PMID 18432196... -
Trimmer Lab NeuroMab Collection
TypeCollection...Human Mouse 190519 Kv2.1 K+ channel scFv [L83/11] L83/11 scFv Kv2.1 K+ channel Rat Mouse 190520 KCC2 scFv...Human Mouse IgG2a 190332 Anti-Kir2.4 K+ channel [N465/11] Kir2.4 K+ channel Mouse Mouse IgG2a 199396 Anti-... -
27 Hot Plasmids from 2016
TypeBlog Post...genes with a bidirectional promoter, integrate at 11 specific chromosomal locations, and stably express... -
Adenovirus Guide
TypeGuide... circumvention strategies . Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372... -
Lentiviral Vector Guide
TypeGuide...Conditional Packaging System. Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463... -
Adeno-associated virus (AAV) Guide
TypeGuide...integration . Current Opinion in Molecular Therapeutics, 11 (4), 442–447. PMID: 19649989 (Link opens in a new...