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...mechanobiology: seeing is believing." Annu. Rev. Biomed. Eng. 10 (2008): 1-38. PubMed PMID:18647110. Additional...spectral properties upon exposure to a specific wavelength of light. PA-FPs can be activated from low fluorescent...brighter fluorescent state by exposure to a specific wavelength of light. The switch or photoconversion usually...multiple times by activating with two distinct wavelengths of light (see Table 2). The best known reversible...photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light, causing emission of green fluorescence...another group of reversible PA-FPs. These were engineered to be the first reversible, monomeric red fluorescent...dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein...

...rapidly became the most popular genome engineering approach. Engineered CRISPR systems contain two components...modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s have ...Xie, H., Liu, D., Mo, H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9...scientists have modified for a wide range of genome engineering techniques . Collectively, these techniques are...R epeat) technologies. Before CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) ...mutation within the targeted gene. However, the strength of the knockout phenotype for a given mutant cell... the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve editing more ...

... Scott Lenaghan 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036...CRISPR Daniel Voytas 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036... molecular biology technologies: from genome engineering and gene expression to reporter systems. Addgene...ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning System Plasmids for rapidly assembling...based on the Golden Gate method. Golden Gate (GreenGate) Jan Lohmann 1000000076 GoldenBraid 2.0 Kit Plant...biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts. Golden Gate (GoldenBraid...MoClo Plant Parts III: Transformation & Genome Engineering Kit Collection of MoClo Level 0 parts for plant...

...specific genes Oligomerized Pool ENgineering (OPEN) Reagents - Engineer zinc finger arrays via OPEN method...method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular Assembly method ...nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium...promote continued research and development of engineered zinc finger technology. Consortium members Keith...have deposited at Addgene various reagents for engineering and expressing zinc finger proteins for distribution...reagents are provided below: Zinc Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes...

...gene expression, reporter plasmids and genome engineering of bacteria....Controlled Expression Reporter Plasmids Genome Engineering You may also like… Genetic Code Expansion Collection...organized by color and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains...#1000000005, 1000000010) contain plasmids and an engineered strain for a B2H system used to screen the activity...112197 pANY3 Temperature (42 °C) Escherichia coli Yingfeng An Additional Addgene Controlled Expression Resources...contains specific bacterial promoters that vary in strength and that can be used to reliably drive expression...Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence (GFP:mRFP1 ratio) Escherichia coli...

...crRNA(SOD1) SOD1 hSyn1 ALS Feng Zhang 109322 pU6-crRNA(TBK1) TBK1 hSyn1 ALS Feng Zhang 109323 pU6-crRNA(TARDBP... FXN EF1a Friedreich ataxia Feng Zhang 142079 TFORF2441 FUS E1Fa ALS Feng Zhang 142463 TFORF0142 FXN E1Fa...E1Fa Friedreich ataxia Feng Zhang 142874 TFORF1856 NR4A2 E1Fa Parkinson's Feng Zhang 142875 TFORF1857 ...TFORF1857 NR4A2 E1Fa Parkinson's Feng Zhang 142944 TFORF2440 FUS E1Fa ALS Feng Zhang 143027 TFORF0449 DNMT1 E1Fa...sensory neuropathy type IE Feng Zhang 143542 TFORF1771 TBP E1Fa Parkinson's Feng Zhang 143543 TFORF1772 TBP...Parkinson's Feng Zhang 143702 TFORF0450 DNMT1 E1Fa Hereditary sensory neuropathy type IE Feng Zhang 144906...telangiectasia Sika Zheng 228918 sg_Atm_i5-ipUSEPR-TR657 ATM TagRFP CMV Ataxia telangiectasia Sika Zheng 228939 pMRX-IP-EGFP-PA-BAX...

...Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish...plasmids and strains used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method. This kit consists...). The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish...of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

...Genome Engineering TALENs TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide...Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL...Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents Assembly via serial ligation. Validated... cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly Kit Assembly by ...cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors...domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena...three 0.5 TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein...

...none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Hongkui Zeng 140137 pAAV-EF1a-iFlpV...pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg* Esteban Engel , Alexander Nectow 69570 pAAV-EF1a-N-CretrcintG ...Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Hongkui Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP...AAV phSyn1(S)-DreO-bGHpA Syn none 5, rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s...51669 AAV phSyn1(S)-FlpO-bGHpA Syn none rg* Hongkui Zeng 174378 pAAV-syn-Flpo Syn none rg* Ian Wickersham...pAAV-EF1a-iFlpV EF1a none 1, PHPeB Hongkui Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre...

...Arrays Zinc Finger Consortium: Zinc Finger Arrays Engineered Zinc Finger Arrays for Endogenous Zebrafish Gene...Targets Recent published reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools... Joung, Randall Peterson, and Joanna Yeh are engineering zinc finger arrays targeted to various zebrafish...Addgene--the specific vector to use depends on the length of the spacer sequence between the half-sites (...zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full Target Site (5' to 3') Left half-... publication. References Rapid 'open-source' engineering of customized zinc-finger nucleases for highly...301. PubMed PMID 18657511 . Oligomerized pool engineering (OPEN): an 'open-source' protocol for making ...

...81. Loren Looger Calcium NEMO calcium sensors Engineering of NEMO as calcium indicators with large dynamics...indicator for two-photon imaging at fiber laser wavelengths. Nat Methods. 2020 May 25. pii: 10.1038/s41592...indicators. Cell Rep Methods. 2023 Jul 27;3(8):100544. Peng Zou Calcium Near-infrared indicator iGECI A near-infrared...dynamics of ATP in single cells. Angew Chem Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ...neurons, astrocytes) A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface...insect expression (Cre-dependent) cAMPr: A single-wavelength fluorescent sensor for cyclic AMP. Sci Signal...measurement Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. Science...

...SynBio Assembly Standards SynBio Blog Genome Engineering SynBio Depositing Labs Hal Alper J. Chris Anderson...Weiss Synthetic biology describes the rational engineering of biological systems for novel functions. Addgene...sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain functional...Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular... in a new window) — International Genetically Engineered Machine Foundation Registry of Standard Biological... opens in a new window) — Synthetic Biology Engineering Research Center The PLOS Synthetic Biology Collection...

...recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana Gradinaru's ...distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana Gradinaru...interest, and model organism under study. *Many engineered AAVs for mice use LY6A to cross the blood-brain...2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Control Feng 163505 CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA...pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3...

...luciferases require different substrates and detection wavelengths. Firefly luciferase is brighter than Renilla ...luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly...Lentiviral expression of firefly luciferase Linzhao Cheng 74444 pLenti.PGK.blast-Renilla_Luciferase Renilla...EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return to Top Reporter Constructs Already ... Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with altered emission...substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine with significantly...Nonsense-mediated decay reporter from Baird et al., 2018 . Engineered circular RNAs for enhanced NanoLuc® protein production...

...pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-9-ALL864 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-5-PV2509 29777...pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-9-ALL856 100048-AAV9 pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-8-PV0142 105534-...51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-1-PV0102 105531-AAV1 pAAV.CMV.LacZ.bGH Control...51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH...51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-9-PV0101 105530-AAV9 pAAV.CMV.PI.EGFP.WPRE.bGH...

...Collection Chemogenetics plasmids encode genetically engineered receptors that are activated by specific small...control signaling within cells expressing these engineered receptors. Chemogenetic receptors are popular...behavior. Depending on the signaling pathway the engineered receptor is coupled to and the specific cell ...are G-coupled protein receptors that have been engineered to respond to specific physiologically inert ...while hM4D(Gi) and KORD are inhibitory. PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains...

... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR...Cas9 Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation... References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran FA, Cox.... 2013 Oct 10;155(2):479-80. PubMed . Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD,...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD...Xavier RJ, Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440...

...emit a specific wavelength of light after excitation by light of a specific wavelength. While more expensive...monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin repeat proteins... constant region. Minibodies - Minibodies are engineered antibody fragments consisting of two scFvs bound... in a range of colors activated by different wavelengths, allowing for easy differentiation between multiple...recognizes and binds to the protein of interest. Signal strength is determined by how many antibodies bind the ... break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may need to be optimized...

...Purification Neurophysiology Cell Ablation Genome Engineering Labeling Overexpression Tracers Tetracycline ...synaptophysin-EGFP for labeling of axon terminals. 1 Hongkui Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby...Flp recombinase. 5 Kazunari Miyamichi Genome Engineering Tools These AAV encode Cas9 nuclease used in ...codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang 85450 pAAV-RSV-SpCas9 RSV-driven, constitutive...AAV-HAPLN1 hSyn-driven, constitutive Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP...

...explored. A tRNA is engineered to recognize the reassigned codon. An aaRS enzyme is engineered to specifically...natural codons are already used, this can be challenging. The most common GCE method involves using a ...load the new tRNA with the ncAA. Together, the engineered tRNA and the aaRS enzyme form an orthogonal pair...machinery. A synthetase plasmid expressing the engineered tRNA and its cognate aaRS enzyme evolved to incorporate...the ncAA in the media to make sure that the full length protein is only made when the ncAA is included....TGA stop codons with the synonymous TAA, and engineering RF2 and tRNATrp to mitigate native UGA recognition...