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...enhancer plasmids for fluorescent labeling and recombination across brain regions and populations created...ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population Region 230551 pAAV-AiE2638m-minBG-SYFP2...pAAV-AiE2563m-minBG-SYFP2-WPRE3-BGHpA AiP1969 AiE2563m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2403m-minBG-SYFP2-WPRE3-BGHpA AiP1772 AiE2403m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA AiP2107 AiE2664m SYFP2 Entorhinal area - L3 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-iCre(R297T)-BGHpA AiP20169 AiE2664m Cre Entorhinal area - L3 pyramidal neurons Hippocampal formation...minBG-SYFP2-WPRE3-BGHpA AiP15079 AiE0127m_3xC2 SYFP2 Entorhinal area - L5b neurons Hippocampal formation 230556...

... to enhance protein solubility and to direct recombinant proteins to the periplasmic space between the... to the periplasm reduces bacterial protein contamination and facilitates extraction. Browse our most ...NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization...gram-negative bacteria with different purification tag combinations, including PelB tags for localization to the...) Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 78577 pL99 PnitA-NitR ε-caprolactam Escherichia...Hypoxia Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 188974 pREDusk-AmpR-MCS FixK2 Red light... Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence (GFP:mRFP1 ratio) Escherichia...

...CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger ...factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1...PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...to activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...

...methods and quality control processes for purified recombinant antibodies....webpage for further information. Production For recombinant antibodies (rAb) encoded by mammalian expression... buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline ...sample is adjusted by diluting or concentrating to a final concentration of 0.9–1.1 mg/mL. Antibody concentration...software and a standard curve is generated. The final antibody concentration is calculated from the standard...performed in parallel with a previous lot of recombinant antibody or the hybridoma-derived equivalent ... Residual Plasmid Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified...

...) N-terminal Anti-MECP2 (M7443) mouse antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2...syndrome can be inherited. In females, random X inactivation results in approximately half of cells expressing...expressing mutant MECP2 . Skewing of these X inactivation ratios can affect disease severity. The MECP2...several functional domains, including: the N - t erminal D omain (NTD) the M ethyl B inding D omain (MBD...signal (not essential for localization) the C - t erminal D omain (CTD) The most common missense mutations...antibody from Sigma (Link opens in a new window) C-terminal Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling...direction and degree of skewing of X-chromosome inactivation. J Med Genet . 44, 148–152. (Link opens in a...

...of the tet-off transactivator (tTA). 2 Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter... loop for amplified tTA expression. 1 Viviana Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible... loop for amplified tTA expression. 1 Viviana Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible..., Tet-inducible expression of EYFP. 1 Viviana Gradinaru 175274 pAAV-rtTA Syn-driven Doxycycline-inducible...tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Hongkui Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby... and synaptophysin-mRuby for labeling of axon terminals. 1 Liqun Luo 60658 pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato... of neurons through a process reliant on Flp recombinase. 5 Kazunari Miyamichi Genome Engineering Tools...

...and shRNA against p53 in different gene/insert combinations A more efficient method to generate integration-free...NR5A2, and microRNA 302/367 in three different combinations of reprogramming factors Efficient germ-line...40. Hochedlinger MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS... and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells induced Beta Cells Adenoviral Mouse Long-...pancreatic beta cells generated by lineage conversion of acinar cells. Nature Biotechnology. 2014 Dec;32(12):1223... of mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):...

...al., 2010; Heinz et al., 2011), engineered to eliminate binding of endogenous mammalian transcription ... by codon optimization of their sequence and eliminating interactions with endogenous factors. Experimental...2017 ) for other viral tTA plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from...AAV vector for dox-inducible expression of Cre recombinase. Takuji Iwasato 63704 pRetroX GFP T2A Cre Retroviral... for dox-inducible expression of GFP T2A Cre recombinase. Floris Foijer 165457 AAVS1-Neo-TRE-CMV-Cre-rtTA...-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible...Dox-inducible expression of a light-activated Cre recombinase. Jacco van Rheenen 104102–104112 TetBow Series of plasmids...

... upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads ...familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic mutations...Huntington’s disease (HD) is a fatal autosomal dominant genetic disorder that causes the progressive degeneration... gene and late-onset AD. It is more likely a combination of gene variation and lifestyle or environmental...exocytosis with VAMP2/3 plasmids to study CNS myelination. (Lam et al. Nat Commun. 2022) Endogenously tag...Innovation Antibody Collection . Explore the recombinant monoclonal antibodies and scFvs for neuroscience... tools and emphasizes their rapid and broad dissemination to the research community. This collection highlights...

...or C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila cells 3.2 MB C terminal tagging...deposited a plasmid which introduces an N- or C- terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes...untagged SpCas9 (Addgene 41815 or #44719 ) in combination with gRNA_AAVS1-T2 (Addgene #41818) or using ...modification. This system can be used to create C- and N-terminal epitope tags. The plasmids in the following articles...selection markers are available. An improved FLP recombinase expression vector allows for efficient marker...

...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...990 KB) Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2...Initiation of Otx2 expression in the developing mouse retina requires a unique enhancer and either Ascl1 or ...Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification...

...nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created... of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA ...modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions... Cas9 is fused to activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing...

....eB Viviana Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 116869 pAAV-...Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Viviana Gradinaru 100896 pAAV.GFA104.PI.eGFP.WPRE.bGH GFA104 EGFP...Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Viviana Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet ...pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Viviana Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre...pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Viviana Gradinaru 137131 pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent...

...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

...PRISM begins with the delivery of stochastic combinations of cell-filling antigenically distinct protein...tags (or "bits"), creating over 100,000 unique combinations detectable by antibodies. All of the PRISM protein...pooled AAVs delivered in vivo generates unique combinations of protein bits. B) Multiplex imaging of barcodes... ... Rodriques, S.G., & Payne, A.C. (2025). Combinatorial protein barcodes enable self-correcting neuron... in a new window) More Resources at Addgene: Recombinant Antibodies for Epitope Tags and Other Markers...

...factors and the sensor behavior may differ from the original characterization (e.g. in vitro vs. in vivo, cytoplasmic...Zhou Calcium Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids...Verkhusha Calcium Small near-infrared indicator iGECInano Design and Initial Characterization of a Small...biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7....charges in live cells. Nat Biotechnol. 2017 Mar 13. Katharina Gaus Endo/Exocytosis Superecliptic pHluorin fused...-pHluorin Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal ...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy...

...Bacterial Expression mCardinal 604 659 17 5.3 27 min Prone to dimerization mCardinal-N1 - Mammalian Expression...Expression mCardinal-C1 - Mammalian Expression mCardinal-pBAD - Bacterial Expression mMaroon1 609 657 8.8...FPbase (Link opens in a new window) or plasmids' original publications. Note for all tables: Brightness ...Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry...

... Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-...Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou...Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley ...Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

...17800 pM-ErbB4-delta-kinase ERBB4 GAL4-BD ALS Marius Sudol 17801 pM-ErbB4-delta-kinase-PY1 mutant ERBB4 GAL4... neuron disease Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS, motor neuron disease Trina Schroer 51412 CMV-p150... GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD ALS Marius Sudol 17803 ... CMV Parkinson's Mark Cookson 25071 2XMyc-LRRK2-Kinase LRRK2 Myc CMV Parkinson's Mark Cookson 25072 2XMyc-LRRK2...ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1...ERBB4 CMV ALS Yardena Samuels 29544 pCDF1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29591 426Gal-FUS...type IE Arthur Riggs 37057 GFP-SMN SMN1 GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1...

... large-scale production. In order to produce recombinant adenoviral vectors, researchers generally need...interest, the ITRs, and homology regions for recombination with the adenoviral plasmid. An adenoviral plasmid...for viral production and homology regions for recombination with the shuttle plasmid. This collection provides...Description PI 16398 BJ5183 E. coli strain for recombination between shuttle plasmid and adenoviral plasmid...