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We narrowed to 1,035 results for: LAS

Showing: 901 - 920 of 1035 results

  1. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...search the tables below for CRISPR Transposase plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Last reviewed on: January 30, 2025 Do you have suggestions for other plasmids that should ...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...integrated along with the donor DNA. The two main classes of CASTs are type I and type V-K. Type I systems... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...

  2. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...Repeat Plasmids Individual REAL TALE Repeat Plasmids You may also like... Keith Joung Lab plasmids Daniel...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...Daniel Voytas Lab plasmids The Joung Lab recently described a rapid and robust protocol for constructing ... Biotechnol. 2011 ). REAL utilizes a set of 28 plasmids each encoding a single TALE repeat domain and ...used for ligation into longer arrays. These 28 plasmids are available individually or as a set as part...wish to join the TALengineering newsgroup . ID Plasmid 32250 TAL006 32251 TAL007 32253 TAL009 32254 TAL010...

  3. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...(AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral...with the plasmid that encodes the viral genome (typically called cis -plasmid or transfer plasmid). Helper...Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV packaging...table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table... Viral Plasmids Adeno-associated Virus (AAV) Plasmids Adeno-associated Virus...CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated viruses (AAV) are small...flexibility. AAV Packaging Plasmids The following tables contain a list of packaging plasmids that are useful to...

  4. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that create double strand breaks in DNA. CRISPR...

  5. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...search the tables below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR... CRISPR Nick CRISPR Plasmids: Nick Browse...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  6. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...mEGFP NA Cytoplasm 114405 ATP2A2-mEGFP AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...

  7. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... Plasmid Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease...Disease Plasmid Resource MJFF Plasmids Shared Research Tools (Link opens in a new window) The Michael J.... Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants ...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to top MJFF... Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...

  8. iPSC Neurodegenerative Disease Initiative Plasmid Collection

    Type
    Collection
    ...phenotyping, and distribution efforts. Plasmids The following plasmids can be used to create cell lines with...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here. Depositor...Also Like... CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research... with the research community. To this end, all plasmids that result from the project efforts will be made...made available for research purposes in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI... cell types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link opens...

  9. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...produces high-quality viral vector preps from select plasmids in our collection. We know that viral vector production... production is a common step after requesting plasmids from Addgene, and by providing this service, we...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...inclusion is what gives you permission to use the plasmid....

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you...yeast transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection...

  11. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7...Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid SNAP-tag...Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid CLIP-tag...Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid ACP/MCP-tag... of proteins inside living or fixed cells. The plasmids associated with this technology are available ...

  12. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish...Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous human...RNA-Guided Nuclease (RGN) Plasmids Genome... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools...Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) for use in mammalian cells...human genes Guide RNA expression plasmids for endogenous zebrafish genes...

  13. CRISPR Guide

    Type
    Guide
    ...., Almeida, R., Gilbert, L. A., Whitehead, E. H., La Russa, M., Tsai, J. C., Weissman, J. S., Dueber, ...different classification of nucleotide base (purine-to-pyrimidine and vice versa) The first classes of base...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

  15. Weighing Reagents Protocol

    Type
    Protocol
    ...Balance Scoopula or spatula Container (beaker, tube, flask, etc.) Reagents Material that will be weighed out...

  16. Centrifugation

    Type
    Protocol
    ... familiar with your lab’s instrument before use. Last Update: September 2022 Equipment Centrifuge Microfuge...

  17. Pipetting Protocol

    Type
    Protocol
    ...correct pipette and pipette tip and using the pipette. Last Update: September 2022 Video Watch the video for...

  19. Kit Free RNA Extraction

    Type
    Protocol
    ...this blog post on RNA extraction without a kit . Last Update: April 10, 2020 Equipment Refrigerated microcentrifuge...

  20. Molecular Biology Reference

    Type
    Guide
    ...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains ...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description Origin...biology basics and plasmid components. Plasmids 101: Origin of Replication Plasmids 101: The Promoter ...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...guide on molecular cloning techniques . Plasmid Elements Plasmids used by scientists today come in many ...
Showing: 901 - 920 of 1035 results