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We narrowed to 1,074 results for: CAN

Showing: 81 - 100 of 1074 results

  1. Caltech Systemic Capsids

    Type
    Collection
    ...was developed at the CLOVER Center at Caltech and can help guide your decision. Figure 1: Decision tree...AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1...al. (2022). Text version of the AAV decision tree can be found at the bottom of this page. For more in ...

  5. Ras Pathway

    Type
    Collection
    ...membrane. Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate ... however when mutations occur in the Ras gene it can be stuck in a constitutively active form, constantly... leading to cancer. Constitutively active Ras is the most common oncogene in human cancer, found in around...National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Color is used...National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Return to top...Targeting RAS signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed ...active Ras is the most common oncogene in human cancer. Cancer...

  6. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  7. Antibody Guide

    Type
    Guide
    ...reaction. Many antibodies can be purchased already conjugated, which can save significant time in the lab. However...choice, you can conjugate antibodies in the lab. The complexity of this can vary significantly based on ... B cell can clone itself to enable higher production of a single antibody, but each B cell can only produce... and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ...that you can detect in a single assay is limited by the number of signaling molecules that can be distinguished...may be present in. However, polyclonals can vary significantly from lot to lot, as immune responses have...

  8. Institute for Protein Innovation

    Type
    Collection
    ... Browse Antibody Plasmids The following plasmids can be used to create or express plasmid-based antibodies... new window) Glypican Antibodies Glypicans are a family of highly conserved proteoglycans crucial for ... includes anti-glypicans that accurately detect glypican presence and explore glypican function, providing...Institute for Protein Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies...families of cell surface and secreted proteins (glypicans and integrins) and common epitope tags, as well...scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description...

  11. TALEN Engineering

    Type
    Collection
    ...FLASH, including detailed and up-to-date protocols, can be found on the TALengineering website. Users may...

  12. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... from Addgene, and by providing this service, we can save you time and help you get results faster. Choose...! Browse AAV | Browse Lenti Packaged on Request Can't find what you need in our in-stock preps? Our Packaged...

  13. Molecular Biology Reference

    Type
    Guide
    ... carry genes and can replicate independently from chromosomal DNA. Although they can be found in archaea...promoter sequence. The promoter can also direct cell-specific expression, which can be achieved by a tissue-specific...plasmid, you can easily make an endless number of copies of the plasmid using bacteria, which can uptake plasmids...in many species and can be useful for a diverse set of applications - Plasmids can drive gene expression...eukaryotes, they play the most significant biological role in bacteria where they can be passed from one bacterium...host, such as antibiotic resistance. This benefit can be context-dependent, and thus the plasmid exists...recombinant DNA, in which many identical DNA fragments can be generated. The combination of a DNA fragment with...

  14. Cloning

    Type
    Guide
    ...enzyme target sites can be repeated throughout a specific DNA sequence, which can make it difficult at...the DNA sequence and can be time consuming compared to other cloning methods. You can find a protocol for...and discovery. With molecular cloning scientists can amplify and manipulate genes of interest and then...complementary overhangs, or which are both blunt-ended, can then be fused together during a ligation reaction...contain several popular restriction enzyme sites. You can easily move (subclone) any piece of DNA that already...the 5’ and 3’ ends of DNA sequence. This fragment can then be cloned into a gateway donor plasmid which...fragment has been cloned into a donor plasmid, it can be rapidly shuttled into any compatible Gateway® ...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater...Plasmid Can be packaged ONLY by a second-generation packaging system that includes the tat gene Can be packaged...continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various genes...have been engineered into lentiviral vectors that can be safely used in laboratory settings. These engineered...variety of cell types and models. Lentiviral vectors can target non-dividing cells, enable stable long-term...split into two plasmids. Although safer, this system can be more cumbersome to use and result in lower viral... contains env Third-generation transfer plasmids can be packaged by either second- or third-generation...

  17. Plan Your Experiment

    Type
    Guide
    ... line that can be transfected at high efficiency Lentiviral transduction Cas9 and gRNA can be present ...components. Selecting a specific genetic manipulation can be a good way to narrow down which reagents are appropriate...efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere... Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha, CBh) or inducible...lines Useful for difficult to transfect cell types, can also be used in vivo A common choice for conducting...component expression may decrease off-target effects Can be used to generate transgenic embryos Cas9-gRNA ...protein product is produced. Alternatively, gRNAs can be designed to target exons that code for known essential...

  18. CRISPR Guide

    Type
    Guide
    ...monocytogenes , which can inhibit the CRISPR activity of both LmoCas9 and SpCas9. Acr proteins can be used to control...desired edit can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR .... CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling can be done by fusing...capabilities , as it can process multiple gRNAs under a single promoter, and can cleave ssDNA non-specifically...and quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2...shredder” system that can cause large genomic deletions. The boundaries of these deletions can be defined, either... an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble- S trand B reak, ...

  20. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater...proto-oncogenes. This can lead to activation of the oncogene and possible development of cancer. Pseudotyping ...continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various genes...been engineered into gamma-retroviral vectors that can be safely used in laboratory settings. These engineered..., or shRNA of interest into the transfer plasmid can be done with most standard cloning methods, including...Production Using HEK293T Gamma-retroviral vectors can be packaged directly in human embryonic kidney 293T...different envelopes to modify tropism, as the env gene can easily be changed. The HEK293T cells will produce...
Showing: 81 - 100 of 1074 results