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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,080 results for: CAN

Showing: 81 - 100 of 1080 results

  1. Caltech Systemic Capsids

    Type
    Collection
    ...was developed at the CLOVER Center at Caltech and can help guide your decision. Figure 1: Decision tree...AAV1-X1 do not display strain-specific tropism and can be used in LY6A-nonpermissive mouse strains. **AAV1...al. (2022). Text version of the AAV decision tree can be found at the bottom of this page. For more in ...

  4. Ras Pathway

    Type
    Collection
    ...membrane. Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate ... however when mutations occur in the Ras gene it can be stuck in a constitutively active form, constantly... leading to cancer. Constitutively active Ras is the most common oncogene in human cancer, found in around...National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Color is used...National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research Return to top...Targeting RAS signalling pathways in cancer therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed ...active Ras is the most common oncogene in human cancer. Cancer...

  6. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety of Cas-containing plasmids...

  7. Institute for Protein Innovation

    Type
    Collection
    ... Browse Antibody Plasmids The following plasmids can be used to create or express plasmid-based antibodies... new window) Glypican Antibodies Glypicans are a family of highly conserved proteoglycans crucial for ... includes anti-glypicans that accurately detect glypican presence and explore glypican function, providing...Institute for Protein Innovation Antibody Collection Glypican Antibodies Integrin Antibodies Epitope Tag Antibodies...families of cell surface and secreted proteins (glypicans and integrins) and common epitope tags, as well...scientists worldwide. Figure courtesy of IPI Browse Glypican Antibodies ID Recombinant Antibody Description...

  10. TALEN Engineering

    Type
    Collection
    ...FLASH, including detailed and up-to-date protocols, can be found on the TALengineering website. Users may...

  11. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... from Addgene, and by providing this service, we can save you time and help you get results faster. Choose...! Browse AAV | Browse Lenti Packaged on Request Can't find what you need in our in-stock preps? Our Packaged...

  15. Synthetic Biology - Overview

    Type
    Collection
    ...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...

  16. Antibody Guide

    Type
    Guide
    ...reaction. Many antibodies can be purchased already conjugated, which can save significant time in the lab. However...choice, you can conjugate antibodies in the lab. The complexity of this can vary significantly based on ... B cell can clone itself to enable higher production of a single antibody, but each B cell can only produce... and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ...that you can detect in a single assay is limited by the number of signaling molecules that can be distinguished...may be present in. However, polyclonals can vary significantly from lot to lot, as immune responses have...

  17. Molecular Biology Reference

    Type
    Guide
    ... carry genes and can replicate independently from chromosomal DNA. Although they can be found in archaea...promoter sequence. The promoter can also direct cell-specific expression, which can be achieved by a tissue-specific...plasmid, you can easily make an endless number of copies of the plasmid using bacteria, which can uptake plasmids...in many species and can be useful for a diverse set of applications - Plasmids can drive gene expression...eukaryotes, they play the most significant biological role in bacteria where they can be passed from one bacterium...host, such as antibiotic resistance. This benefit can be context-dependent, and thus the plasmid exists...recombinant DNA, in which many identical DNA fragments can be generated. The combination of a DNA fragment with...

  18. Plan Your Experiment

    Type
    Guide
    ...transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be low, but ...within the gene itself can also be effective, as the Cas9 bound to the DNA can interfere with transcription...gRNAs can save your lab valuable time and resources when carrying out CRISPR experiments and can be used...transfection or electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid...targeted edits can lead to changes in more than just the DNA sequence. If this is the case, you can confirm ...different Cas proteins available, and researchers can adapt these experiments for different organisms. ... to have a biological question in mind. Then you can decide how to answer that question using genetic ...

  19. Cloning

    Type
    Guide
    ...enzyme target sites can be repeated throughout a specific DNA sequence, which can make it difficult at...the DNA sequence and can be time consuming compared to other cloning methods. You can find a protocol for...and discovery. With molecular cloning scientists can amplify and manipulate genes of interest and then...complementary overhangs, or which are both blunt-ended, can then be fused together during a ligation reaction...contain several popular restriction enzyme sites. You can easily move (subclone) any piece of DNA that already...the 5’ and 3’ ends of DNA sequence. This fragment can then be cloned into a gateway donor plasmid which...fragment has been cloned into a donor plasmid, it can be rapidly shuttled into any compatible Gateway® ...

  20. Retrograde AAV viral preps

    Type
    Collection
    ... neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent...lab. Results for our quality control experiments can be found on the material pages for viral catalog ...
Showing: 81 - 100 of 1080 results