We narrowed to 1,071 results for: CAN

...insert the viral genome into a host cell. Scientists can take advantage of this to stably integrate transgenes...

... neurons. Specific classes of projection neurons can be targeted by using this serotype to deliver Cre-dependent...lab. Results for our quality control experiments can be found on the material pages for viral catalog ...

...with dual labeling when used with two substrates Can be used in live or fixed cells, and for internal ...

...preps that encode site-specific recombinases, which can be used to control gene expression. See our Cre-lox...

... mutations in Cas9 to enhance nuclease activity; can be used in PE2–PE5 systems PE6 — small prime editors...

... at one of six hotspot residues. These mutations can be inherited or arise through exposure to mutagens... Cancer Research p53 Pathway p53 Pathway You May Also Like: Cancer Pathway ORF Kit mTOR...SDS-PAGE gel. Cancer Impact p53 is the most frequently mutated gene (>50%) in human cancer; most of these...mechanisms by which mutant p53 is known to function in cancer. p53 Pathway Plasmids Click on a name to find available...component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2...suppression. Bieging KT, Mello SS, Attardi LD. Nat Rev Cancer. 2014 May;14(5):359-70. PubMed PMID: 24739573 ....the mutant p53 field. Brosh R, Rotter V. Nat Rev Cancer. 2009 Oct;9(10):701-13. PubMed PMID: 19693097 ....

...University of Florida. References for each serotype can be found below under Citations. AAV2(Y444F) The AAV2...

...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed...conditions. With proper experimental design, biosensors can enable investigation of a signaling pathway or measurement...an existing construct, as fluorescence properties can be affected by environmental factors and the sensor...1147-1155. Lin Tian Endocannabinoid GRAB_eCB2.0/eCBmut biosensor for endocannabinoids in vivo (Constitutive...peroxide Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based...sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo. Nat Biotechnol. 2021 Nov ...

...fluorescent proteins under general promoters. These AAV can be used to compare the activity of different serotypes...

...small proteins that can be secreted by immune cells and other cell types. They can be grouped in to the...cells will expand and give rise to memory cells that can recognize a recurrent infection and rapidly initiate...duration of signaling controls the fate of B cells and can lead to the formation of a high avidity BCR through...described. NK Cell Signaling Natural killer (NK) cells can kill cells that have been infected by a foreign pathogen...cytokines when cleaved from the cell membrane and can also function as signaling proteins involved in proliferation...

... information is available for each construct and can be accessed using the links to view individual plasmid...N., Black, S. F., Bonaguro, R. J., Brauer, E., Candido, T. R., Castellarin, M., Chen, J., Chen, Y., Cheng...

...Packaging Service: Optogenetics AAV Optogenetic tools can be used to control cell activity in living tissue...

...reaction. Many antibodies can be purchased already conjugated, which can save significant time in the lab. However...choice, you can conjugate antibodies in the lab. The complexity of this can vary significantly based on ... B cell can clone itself to enable higher production of a single antibody, but each B cell can only produce... and can be used to increase flexibility or specificity in antibody-based methods. Antibodies can recognize...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ...that you can detect in a single assay is limited by the number of signaling molecules that can be distinguished...may be present in. However, polyclonals can vary significantly from lot to lot, as immune responses have...

... enzyme target sites can be repeated throughout a specific DNA sequence. This can make it difficult at...no homology ends can still be fused in a seamless manner. To accomplish this, you can transform into yeast...and discovery. With molecular cloning, scientists can amplify and manipulate genes of interest and then...complementary overhangs, or which are both blunt-ended, can then be fused during a ligation reaction with T4 ...usually within the multiple cloning site (MCS). You can easily move (subclone) any piece of DNA that already...leaves behind a short scar in the DNA sequence and can be time-consuming compared to other cloning methods...’ and 3’ ends of the DNA sequence. This fragment can then be cloned into a Gateway donor plasmid, which...

... information and can replicate independently from chromosomal DNA. Although they can be found in archaea... and can be specific to a certain species, tissue, or cell type. The strength of the promoter can control...every protein within an organism. Before proteins can be translated, the DNA is converted to RNA in a process... only 20 natural amino acids, so multiple codons can encode the same amino acid — a phenomenon called ... to the host, such as antibiotic resistance, and can be passed from one bacterium to another by a type...recombinant DNA, in which many identical DNA fragments can be generated. The combination of a DNA fragment with...backbone generates a recombinant DNA molecule, which can be used to study DNA fragments of interest, such ...

...transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be low, but ...within the gene itself can also be effective, as the Cas9 bound to the DNA can interfere with transcription...transfection or electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid...targeted edits can lead to changes in more than just the DNA sequence. If this is the case, you can confirm ...different Cas proteins available, and researchers can adapt these experiments for different organisms. ... to have a biological question in mind. Then you can decide how to answer that question using genetic ...but generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi...

...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater...Plasmid Can be packaged ONLY by a second-generation packaging system that includes the tat gene Can be packaged...continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various genes...have been engineered into lentiviral vectors that can be safely used in laboratory settings. These engineered...variety of cell types and models. Lentiviral vectors can target non-dividing cells, enable stable long-term...split into two plasmids. Although safer, this system can be more cumbersome to use and result in lower viral... contains env Third-generation transfer plasmids can be packaged by either second- or third-generation...

...monocytogenes , which can inhibit the CRISPR activity of both LmoCas9 and SpCas9. Acr proteins can be used to control...desired edit can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR .... CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling can be done by fusing...capabilities , as it can process multiple gRNAs under a single promoter, and can cleave ssDNA non-specifically...and quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2...shredder” system that can cause large genomic deletions. The boundaries of these deletions can be defined, either... an enzymatically inactive form of Cas9; can bind, but cannot cleave DNA DSB D ouble- S trand B reak, ...

...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater...proto-oncogenes. This can lead to activation of the oncogene and possible development of cancer. Pseudotyping ...continue their lifecycle and replicate. This feature can be harnessed for stable delivery of various genes...been engineered into gamma-retroviral vectors that can be safely used in laboratory settings. These engineered..., or shRNA of interest into the transfer plasmid can be done with most standard cloning methods, including...Production Using HEK293T Gamma-retroviral vectors can be packaged directly in human embryonic kidney 293T...different envelopes to modify tropism, as the env gene can easily be changed. The HEK293T cells will produce...

...R-mAbs in transfected cells and their validation can be found below. With these plasmids researchers will...Antibody Plasmid Collection The following plasmids can be used to produce R-mAbs from transfected mammalian...More scFv Plasmid Collection The following plasmids can be used to produce scFvs from transfected mammalian...Nanobody Plasmid Collection The following plasmids can be used to produce nanobodies from transfected bacterial...] VGlut1 Rat Mouse IgG2a 128639 Anti-Versican [N351/23R] Versican Mouse Mouse IgG2a 128640 Anti-Pan-Ankyrin... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...