We narrowed to 99 results for: ubc

1. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

2. Antibodies 101: Validation

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   Blog Post

   ...antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently...

3. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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   Blog Post

   ...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...

4. Antibodies 101: Introduction to Antibodies

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   ...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...

5. Delivery Methods for Generating iPSCs

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   ...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...

6. Fluorescent Protein Guide

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   ...that affect signalling pathways within cells. Subcellular Localization Determine where your protein of ...

7. Trimmer Lab NeuroMab Collection

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   Collection

   ...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary...References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

8. Malate Dehydrogenase CUREs Community Collection

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   ...species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal...

9. Allen Institute for Cell Science Plasmid Collection

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   ...CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute...

10. Plant Plasmids and Resources

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    ...GRF4-GIF1 for wheat and other monocots - Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for...

11. TALEN Plasmids and Kits

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    ...expression of fluorescent TAL effectors to visualize subcellular positioning of target DNA sequences in living...

12. Lentivirus Plasmids

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    ...expression; puro resistance Sabatini 14883 FUGW 3rd hUbC-driven EGFP; can be used for cDNA expression Baltimore...

13. Neurodegeneration Research Collection

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    Collection

    ...of fluorescent proteins, including Markers of Subcellular Localization, Optogenetics Plasmids, and Chemogenetics...

14. 27 Hot Plasmids from 2016

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    Blog Post

    ...channel). The Guler lab optimized the actuator’s subcellular localization using trafficking signals and named...

15. CRISPR Plasmids - Empty gRNA Vectors

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    ... Mammalian U6 none S. aureus Joung pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 71236 Mammalian/Lentiviral hU6...

16. Antibody Guide

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    Guide

    ...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...? You may need to buy isotype-specific or even subclass-specific secondary antibodies to allow for differentiation...

17. Cloning

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    Guide

    ...restriction enzyme sites. You can easily move (subclone) any piece of DNA that already has restriction...

18. Chemogenetics Guide

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    Guide

    ...targeted to specific tissues, cell types or even subcellular regions of a neuron. Cell-specific expression...

19. Optogenetics Guide

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    Guide

    ...blue-shifted channelrhodopsin from Platymonas subcordiformis 445 CoChR Channelrhodopsin from Chloromonas...