...expression can be long-lived. The utility of AAV is currently limited by its small packaging capacity (∼4.5 ...

...enzymes and these different Dicer-like enzymes are involved in the biogenesis of different small RNAs (Figure...choice of tool requires careful balancing of the inherent advantages and limitations of any technique in...that function downstream of DNA, such as RNA interference, can reversibly exert their effect independent...independent of gene copy number. RNA interference (RNAi) is a conserved eukaryotic process where approximately...transcriptionally or post-transcriptionally. In plants, different dicer-like enzymes (DCL) process the dsRNA into...equivalently precise, but each method recovered different genes known to be required for viability (Morgens...subsequent two. Consequently, I will focus on different approaches and considerations present at the first...

...sessions The poster session from conference to conference can be wildly different. There can be no presenter...participants used the conference hashtag after the conference was over. Depending on the conference, organizers ...scientific conferences. Plan your sessions, not your walks between sessions Like many large conferences with...can use to help plan your conference experience.One of the biggest differences: you don't have to build..., requiring you to zoom in to different parts of the poster. Conferences, such as Photonics Online Meetup..., 2020 Final thoughts on virtual conferences Virtual conferences remove many barriers of participation...spend with the conference. “If you want to have the same experience as an on-site conference, you really ...

...freezers that the samples can be stored in? Different shelves? Different racks on each shelf? Give each container...about location, location, location There are many different types of samples at Addgene - DNA sent from depositors... that we never run out. There are a number of different strains of E. coli that can be used for transformation... concentration or need to be transformed into different cells than were originally requested. Most of ...analysis from our protocols and molecular biology reference pages! Resources Avaliable on the Addgene Blog...

...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...

... Addgene’s TAL effector and TALEN kits here . References Efficient design and assembly of custom TALEN...

...fused between ECFP and Venus Back to Top FRET Reference Standards The following plasmids have well-characterized...

...green cards and citizenship.   Lauren Celano, CEO Propel Careers. Lauren is passionate about working with...This post was contributed by guest bloggers Lauren Celano of Propel Careers and Rachel Casseus, Esq. ...information presented may not reflect the most current legal developments.  Further, this post may contain...development for foreign national researchers and entrepreneurs who are looking to continue their careers in...tasked with working with US immigration in its current state. As of September 2017, USCIS received 116,224... As an international researcher and aspiring entrepreneur, you have many career options available to you...skills overtime for success. Many people will have different roles, titles, and responsibilities during their...

...We’re excited to welcome Karen Katz, Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors...scientific community manage those plasmids so scientists aren’t bogged down with distributing them and spending... enjoying that immensely. Paratore: I have two children active in sports, so I enjoy watching their games... Daisy, or out on golf course! Learn more about Karen Katz, Esq Ms. Katz is the Director of Intellectual...Property Programs at Suffolk University Law School. Karen graduated with highest honors from the University...associated with Hale & Dorr and Crowell & Morning. Karen was a founding member of Mass Medical Angels, a ...

...codon usage biases of different organisms. Different species have different preferences for the codons they...scientists from MIT tapped into this data to learn about trends in synthetic biology and DNA synthesis. They published...the gene may not express well because of these different codon usage biases. But if a gene’s sequence was...could be determined through comparisons with a reference sequence database. Through a series of simulations...Kunjapur says. Percent sequence identity was a different story - the team found that the percent sequence...sequences in Addgene’s database. They showed a growing trend of sourcing genes from distantly related organisms...and synthetic biology is expanding rapidly.  References 1. Kunjapur, Aditya M., Philipp Pfingstag, and...

...Koeris' own transition from graduate student to entrepreneur, listen to our full interview with Dr. Koeris...contrasts being a graduate student with being an entrepreneur 29:00 - 32:20 : What did It mean for Dr. Koeris...developing a diagnostic? 36:53 - 38:00 : As an entrepreneur, is it more important to develop something that...Board and how he hopes to help young biotech entrepreneurs.   We'd like to thank Dr. Koeris for taking... entrepreneur...

.... I spoke with Dr. Mike Koeris, co-founder and current VP of Business Development & Operations of Sample6...roles and skills necessary to be a succesful entrepreneur? As a co-founder of a biotech startup, Mike ...its pitch the best, wins. He explained that entrepreneurs must clearly communicate their idea at various...be reproducible by someone else. Second, "an entrepreneur must be OK with the constant fear of failure...are only successful if they are 100X better than current technology and get customers to change behavior...

...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...

...on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end ...

...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrh10...

... 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...other and what to do.  Saved by the Tweet - The conference hashtag I had, however, started a Twitter account...at an institution where training opportunities weren’t always available. Even more valuable, however, ...like anyone at a meeting who felt isolated at a conference for any reason was a Singleton. Figure ... gene transfer, which occurs when bacteria of different evolutionary lineages share genes that can improve...

...proliferates and is redifferentiated to a different cell type. Transdifferentiation In this process, differentiated...reversal of a differentiated cell type to an undifferentiated state and then redifferentiation into the cell...variety of plasmids for differentiation here. Transdifferentiation Dedifferentiation to an intermediate pluripotent...ability to convert a differentiated cell to a different cell type. While transdifferentiation is suited for ... are converted to cells of a different lineage, without dedifferentiating into a pluripotent stage. Cellular...pluripotent state. Redifferentiation - Conversion of the pluripotent cells into differentiated cells of choice...control the cell fate of iPSCs and differentiate them into different cell lineages [13]. Over the last ...

...supporting in-person conference attendance, the award now supports virtual conference attendance. We also...Michael Davidson and Roger Tsien Commemorative Conference Awards. Congratulations to the five winners! ...awards help early career researchers attend a conference of their choice while honoring the late Michael...faster and more sensitive than its predecessors. Currently, Aggarwal, and colleagues Filip Tomaska and Tim...CR neurons of the spinal cord. Created with BioRender.com. Image from Haoyi Qiu.   He Tian: Directed...had improved performance in live mouse brains. Currently, she is using the improved voltage indicator coming...

...molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium...