...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...maintained for ~20 passages before being discarded and replaced with a new vial of early passage cells. Approximately...

...or repression. The TCS light sensors described in ACS Synthetic Biology (2014) represent the newest versions...Photobiology: Optogenetics for E. coli. Schmidl, et al. ACS Synth Biol. 2014 Nov 21;3(11):820-31. Inncredible...compartment (e.g. the plasma membrane, the extracellular space, or the lysosomes) can take many paths. Understanding...that is quick, versatile, and robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector,...the CRISPR system. The ease and flexibility of the LACE technology makes this system widely accessible for...thereby creating a single-molecule reporter of intracellular processes. Multimerization via the SunTag system...combination of all 11 att pairs in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable...

...the loxP sites is reversed. Deletion: If the sites face in the same direction, the sequence between the ...Cre fragments (NCre and CCre, respectively) and placed under the control of different promoters. Expression...CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...

...Bacteria: pCas9 new spacer cloning pCas9 PDF 125.6 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR...

... RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...

... Encodes the AAV2 capsid with the N587 residue replaces with a TAG stop codon Mali 130878 pAAV-DJ-N589X...Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon Mali 166921 AAV-F in pAR...

...Far-Red Calcium Sensors HaloCaMP1a HaloCaMP1b Acetylcholine Sensors iAChSnFR Dopamine Sensors dLight RdLight... Promoter Sensor Label Activity Serotype PI Acetylcholine Sensors 137950 pAAV.hSynap.iAChSnFR Syn iAChSnFR...

...be lysed or the substrate must be delivered intracellularly in order to measure luminescence. Gaussia luciferase...Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and...

...Iodixanol Gradient Ultracentrifugation AAV Purification by Iodixanol Gradient Ultracentrifugation You may also...tube at the interface of the 60% and 40% gradient (see Figure 2) with an 18 ga needle. Place the first ...fractions per tube. Avoid the proteinaceous material near the 40–25% interface. Pro-Tip As soon as the top...care to avoid collecting the proteinaceous material at the 40–25% interface. Repeat for each QuickSeal ...Iodixanol gradient after ultracentrifugation. The arrow indicates the 60–40% interface. The vertical black ...Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) ... be completed in one (long) day. Equipment Ultracentrifuge T70i rotor or equivalent Reagents Opti-Prep...

...that was created for systematic screening of extracellular protein interactions to identify novel parasite-parasite...

...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...

...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

... et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter. 46966...

...each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream...

...that was created for systematic screening of extracellular protein interactions to identify novel receptor-ligand...

...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

...structures of proteins of medical relevance, and place them in the public domain without restriction. Many...

...Key plasmid features and pX458 modifications: replacement of Cbh promoter with EF1alpha addition of mCherry...

...single plasmid, multiple orders would need to be placed through our website. Every order will need to be...

...that was created for systematic screening of extracellular protein interactions to identify novel receptor-ligand...