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We narrowed to 1,044 results for: REN

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Showing: 121 - 140 of 1044 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ...Resources Addgene Blog: Rabies and Neuronal Tracing References Wickersham IR, Finke S, Conzelmann KK, Callaway...

  2. Institute for Protein Innovation

    Type
    Collection
    ...antibodies targeting other synaptic cleft proteins are currently under development. Figure 2: Key adhesion proteins...

  3. Antibody Production

    Type
    Collection
    ...sequenced via the Sanger method, and aligned with the parental plasmid sequence to confirm the antibody’s identity...

  5. Antibody Plasmid Collection

    Type
    Collection
    ...engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822. Scheeren and Verdoes Backbones for mouse recombinant antibodies...

  6. mTOR Pathway

    Type
    Collection
    ...Screening Kit (from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease....

  7. Distribution to Industry

    Type
    Collection
    ...table below to see all of the plasmids that are currently available to industry scientists. ID Plasmid Gene...

  8. Promega Plasmid Collection

    Type
    Collection
    ... chemical compound and its target protein. The apparent affinity of test compounds is measured by competitive...

  9. AAV Packaged on Request

    Type
    Collection
    ... scientists sometimes require preps beyond our current in-stock capabilities. To meet that need, our Packaged...

  10. TALEN Guide

    Type
    Collection
    ... Addgene’s TAL effector and TALEN kits here . References Efficient design and assembly of custom TALEN...

  11. Viral Production

    Type
    Collection
    ... 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

  12. TALEN Plasmids and Kits

    Type
    Collection
    ...induces mutation at rates much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs...

  13. Lentivirus Plasmids

    Type
    Collection
    ...integrase-deficient lentivirus. David Rawlings and Andrew Scharenberg 22502 pCEP4-tat N/A Expression of tat . Increases...

  14. Brain Armamentarium

    Type
    Collection
    ...molecular genetic reagents to gain access to many different brain cell types. The expanded collection of Armamentarium...

  15. Brain Initiative Collection

    Type
    Collection
    ...made from a selected set of plasmids that are currently in our repository. AAV are distributed as 100 ...

  16. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...788679 Lin R, Wang R, Yuan J, Feng Q, Zhou Y, Zeng S, Ren M, Jiang S, Ni H, Zhou C, Gong H, Luo M (2018) Cell-type-specific...in decreased efficiency of recombination and preferentially leads to gene constructs where the stop cassette...likely generalizable to other systems as well. References: del Valle Rodríguez A, Didiano D, Desplan C...plasmids at Addgene Read our Molecular Biology Reference ...

  17. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang Z, Mu Z, Chen... investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An... of the scientific research infrastructure. References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S,...(2006) Is there an ideal animal model for SARS? Trends in Microbiology 14:299–303 . https://doi.org/10.1016...

  18. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...10535 (2018). 1 10.1074/jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed...park. Happy editing!   Resources and References References Yu, Y., Guo, Y., Tian, Qigi, Lan, Y., et...overhangs at the break site – an early HR step (Moreno-Mateos, et al., Nat Com). Another method used ...the break, potentially shuttling repair down a different pathway (Charpentier et al., Nat Com).   ...Cas9 delivery. eLIFE. (2014) 10.7554/eLife.04766 Moreno-Mateos, M. A., Fernandez, J. P., Rouet, R., Vejnar...

  19. Cutting Once Is Harder Than It Sounds: Your Guide to Minimizing Off-Target Effects

    Type
    Blog Post
    ...biologist working on improved genome editing at HUN-REN RCNS. He is passionate about sharing science and ...sequences (or spacers) themselves have an inherent differential propensity to cleavage, and this often-overlooked...plasmid cloning problems.  References and Resources References Kulcsár, P. I., Tálas, A., Ligeti...prediction tools do exist for a few IFNs, but there is currently no reliable way to predict the off-target effects...cleaved (cleavability rank). By combining these two trends, the cleavage rule can be established: If a nuclease...Blackjack-HypaR, as these variants are located in different ranges of the activity spectrum (Figure 2). Identify... SpCas9 is below ~5–10%, consider selecting a different target. Remember, if WT SpCas9 does not cleave...

  20. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...-023-02050-w Yi, Z., Zhang, X., Wei, X., Li, J., Ren, J., Zhang, X., Zhang, Y., Tang, H., Chang, X., Yu...) enzymes. Various other proteins included in different CGBE tools allow the researcher to direct the ...component of the nucleoside inosine. Created with BioRender.com. Inosine excision leads to the first adenine...a translesion synthesis polymerase known to preferentially incorporate A bases across from abasic sites...indicate natural cellular processes. Created with BioRender.com. The first deaminase-free base editor allows... Figure 3: Mechanism of gGBE. Created with BioRender.com. Base excision editing extends to thymine...recognize thymine (T) or cytosine (C). Several different teams succeeded with this approach around the ...
Showing: 121 - 140 of 1044 results