...single-cell) approach is to interrogate state with qPCR. The re-arrangement of DNA segments in each state of... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence... prescribed region of DNA termed the “register”. Each chemical input to an RSM drives expression of a ... set of possible downstream states that can be achieved by expression of other recombinases. Multiple ...

...replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no proteins). The chemistry...

...new possibilities for GFP-labeled lines, and they each have their own advantages. T-DOGGs can be made drug-inducible...

...a tool in mammalian cell systems, silencing is achieved through the delivery of a double-stranded RNA ...

...stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity to the spacer...

...of patients results in billions of dollars spent each year. Unfortunately, the causes of most neurodegenerative...

...engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules...

...tagging in your system, use the plasmids as listed in each row. If your own gene of interest is currently unavailable...

...TAGACTTTCCTGAAAAGCaaaaatgctagattttGCCATTTTTGCTGTGACA dachc TAL3234 & TAL3235 TCCAGACCTGGCAGACCTcctaaaaggacccagaATGTGACATCACCCGACA...

...Tools CRISPR technology is a promising therapeutic approach for precise editing of mutations in the MECP2 ...

...table below. Additional information is available for each construct and can be accessed using the links to...

...activation using SAM There are two sets of 3 vectors each available for mammalian endogenous gene activation...

...these plasmids can be transposed into the attTn7 attachment site found in many enterobacterial genomes. The...

...variety of organelles and subcellular structures each with their own unique function. The nucleus, for...

...Fluorescent Protein Fusions (Organized by Color) For each table: Excitation and Emission maximum are listed...

...using a sequencing machine that recognizes the different fluorescent labels. The machine detects which fluorescently...templates, are spatially separated from each other. These attached DNA templates are then amplified again...again producing ~1,000 copies of each template. Each template is then replicated using the modified bases...fluorescent color that is emitted each time a base is added. Again, each base (A,C,T, or G) is labelled ...an organism. Proteins are made up of amino acids. Each amino acid is encoded for by 3 nucleotides termed...20 natural amino acids and 64 codon combinations each amino acid is encoded for by multiple codons. Plasmids...fragments, which is far too many to separate from each other for the purposes of isolating one specific...

...applications common to each category, including an outline of the basic steps needed for each application, important...different isotypes for each primary antibody and secondary antibodies specific to each isotype. Overview of... data. Check that your machine has appropriate lasers and filters for each fluorophore you select. Suggested... itself prior to antibody production; therefore, each B cell only produces one type of antibody. Monocultures...enable higher production of a single antibody, but each B cell can only produce one variable region. Isotypes...-antibody units, while others exist as monomers. Each antibody class therefore has a valency, or number...typically the number of antibodies multiplied by two, as each antibody has two binding sites. Isotype Description...

...specificity. Dual-nickase approach increases specificity but is less efficient. Each putative knockout allele...engineering . Prior to CRISPR, genome engineering approaches like zinc finger nucleases (ZFNs) or transcription-activator-like...rapidly become the most popular genome engineering approach. Engineered CRISPR systems contain two components...experiment, you might consider using the dual nickase approach to create a double nick-induced DSB. The nickase...have been engineered using cell-based selection approaches. Yeast-based screening systems resulted in evolved...termed left & right homology arms .) The length of each homology arm is dependent on the size of the change...several gRNAs from the same plasmid ensures that each cell containing the plasmid will express all of ...

...) until peak expression of the opsin is reached. This approach is especially powerful when combined with...including a brief overview of the wild-type opsin of each and some examples of relevant variants. This list...closing of the channel 470(activ.), 590(inact.) ReaChR Red-activatable variant of CrChR2 590 Channelrhodopsins... variant 467 Aurora Red-shifted anion-selective ReaChR variant 517 Halorhodopsins Jaws Red-shifted, light-driven...Generally, the most robust and stable expression is achieved in a transgenic mouse line, such as for example...that bind to the LOV domain only in the dark. attaching only one member of the Zdk/LOV2 pair to the target...KR, Deisseroth K. 2010 Molecular and cellular approaches for diversifying and extending optogenetics. ...