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Showing: 21 - 40 of 312 results
  1. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ..., Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Plasmid Gene/Insert Promoter...rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead...
  2. Church Lab CRISPR Plasmids

    Type
    Collection
    ...CRISPR Blog Posts Bacteria and archaea have evolved adaptive immune defenses termed clustered regularly interspaced...CRISPR-associated (Cas) systems that use short RNA to direct degradation of foreign nucleic acids. We have engineered ...
  3. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant adeno-...indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant adeno-... the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers Ltd: Nature...proteins VP1, VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang...
  4. Luciferase Plasmids

    Type
    Collection
    ...Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 106292 pLS-SV40-mP-Rluc Renilla Insertion...Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly/Renilla Creation...
  5. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ...Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Jinek M, Chylinski K, Fonfara...
  6. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ..., Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Want more information on the...
  7. Boxem Lab CRISPR Plasmids

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts We adapted the S. pyogenes CRISPR/Cas9 system for use in C...
  8. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...Lifetechnologies Gateway Destination Vectors ) to adapt to different systems by LR clonase reaction. These...
  9. Synthetic Biology - Overview

    Type
    Collection
    ...SynBio Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi...
  10. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...immunoprecipitation (enChIP). Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H. Sci Rep. 2013 Nov ...
  11. TALEN Plasmids and Kits

    Type
    Collection
    ...method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector Engineering Reagents...
  12. Caltech Systemic Capsids

    Type
    Collection
    ...Gradinaru Lab and the Caltech CLOVER Center and was adapted from Challis et al. (2022). Text version of the...
  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging of proteins expressed from their...
  14. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGTCCTCATCCCCGTCCCgccgcggcgccaccgtCAGCTTCCACAACATCAA adam15 TAL3016 & TAL3017 TGTTTCTGGCGGACTCTCgtcaccattcttctgtTTGAAGGCACACCTTTGA...
  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...30905392 Marcinkiewcz CA, Mazzone CM, D'Agostino G, Halladay LR, Hardaway JA, DiBerto JF, Navarro M, Burnham...
Showing: 21 - 40 of 312 results