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Showing: 61 - 80 of 82 results

  2. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents...

  5. Deep Dive: qPCR

    Type
    Blog Post
    ...configurations can help you identify pipetting or evaporation errors. And if time allows, you can fit in a ...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus ...

  8. mTOR Pathway

    Type
    Collection
    ...the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal upstream...

  9. Qi Lab CRISPR Page

    Type
    Collection
    ...protospacer adjacent motif or PAM, sequence: NGG) juxtaposed to the DNA complementary region. The uniqueness...

  13. Western Blot

    Type
    Protocol
    ...BSA standard concentration versus absorbance. Extrapolate the total protein concentration of the sample...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 188214 Anti-GST [N100/13R ] GST Schistosoma japonicum Mouse IgG2a 188215 Anti-TrpC7 [N64A/36R ] TrpC7...GST scFv [N100/13] N100/13 scFv GST Schistosoma japonicum Mouse 190522 Aldh1L1 scFv [N103/31] N103/31 scFv...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...processes such as differentiation, proliferation, and apoptosis. In humans, mutations that permanently activate...

  17. Validated gRNA Sequences

    Type
    Collection
    ...GAAGCGGGCAAAGGGGCGAC 58780 cut/nick S. pyogenes 24954249 Yamamoto apoea D. rerio GGATGAGCCAAGAAGCCGCT 42241 cut S. pyogenes...

  18. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...tuberculosis M. smegmatis Green monkey ( C. sabaeus ) Kaposi's Sarcoma-associated Herpes Virus (KSHV) Cow ( B...

  19. CRISPR Guide

    Type
    Collection
    ...dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus ...

  20. Chemogenetics Guide

    Type
    Guide
    ... 4 ). Additional varenicline analogs, termed ultrapotent PSEMs (or uPSEMs), have also been developed as..., Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research and potential clinical...
Showing: 61 - 80 of 82 results