...the coding strand , meaning that the suffix will need to be reverse complimented for primer design. The...

...below for information on substrates and synthases needed for use of these tags. Plasmids Addgene ID Plasmid...

...hyperactivation of mTORC1 in cancer promotes the conditions needed for uncontrolled cell growth and proliferation,...

... and aging. Scientists can knock down genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9...

... generation time (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it...

...) containing lentivirus is first cleared by low speed centrifugation and then by filtration through a ...

...laboratories performing phosphoproteomic experiments in need of reference spectra or phosphopeptide standards...

...preparation. Systemic Delivery AAV Capsid Choice Need help deciding which capsid to choose? The following...

...drive expression of your favorite gene, you will need a plasmid with a promoter that will be functional...

...this vector contains an insert (MAT2A) which would need to be replaced by the gene of interest. tTA Off ...

...sufficiently proliferated when the concentration exceeds ~100,000 cells/ml or for adherent cells, the cells...

...Already know what gene or regulatory element that you need a luciferase reporter for? Browse or use the search...

...Pluripotent Stem Cells From Adult Blood Cells Under Feeder-Free and Xeno-Free Culture Conditions: A Clinically...

...the stop codon is excised, and gene expression proceeds. Cre-dependent Gene Knockout: Conversely, putting...

...to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage sensor to detect...

...process may need to be optimized for individual assays. Clear cell debris by sedimentation. Proceed with IP..., an antimicrobial agent, can block some amines needed for conjugation reactions and should be removed...chosen. Signal Amplification The amount of signal needed from a specific antibody can vary depending on ...category, including an outline of the basic steps needed for each application, important controls, and any...samples to make proteins available. Tissue samples may need additional processing steps, such as sonication....signal levels. In ChIPseq, an input control will need to be collected before immunoprecipitation. Antibody...from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be...

... there is an ideal approach. This may be due to speed, cost, availability of starting materials or just...compatible ends together. TOPO® cloning thus does not need restriction enzymes or an exogenous ligase providing... of PCR products into donor plasmids without the need for restriction enzyme cloning. For more information...of DNA in the chosen orientation, and without the need for any unwanted sequence at the junctions (such... a seamless manner. To accomplish this, you just need to introduce into the yeast the two (or more) fragments...would like to fuse. A major disadvantage is that you need to be set up to grow, transform and purify DNA from...

...Cas9-gRNA complex binds a putative DNA target, the seed sequence (8-10 bases at the 3′ end of the gRNA targeting...) will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...mismatches between the target sequence in the 3′ seed sequence completely abolish target cleavage, whereas...homology exists. These sites are called off-targets and need to be considered when designing a gRNA for your ...Remember - if you are using a 2-vector system, you will need to transduce cells that are already expressing Cas9...necessary to customize an existing reagent to suit your needs. Genetic Manipulation Application Cas9 gRNA Additional...Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed in your target cells...

...targets for development into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read...back-metabolism of CNO to clozapine, demonstrating the need for alternative DREADD ligands. Compound 21, Deschloroclozapine...while PSAMs have more than one domain, both of which need to be expressed. However, neuronal control through...experiment, an AAV-encoded chemogenetic plasmid may need to be targeted to specific tissues, cell types or...

...the genetic code and provides all the information needed for cells to make proteins and other molecules ...sequencing. To understand Sanger sequencing, we first need to understand DNA replication. DNA is a double helix...pieces of a 2,000 piece puzzle. A scientist would need to run a ton of Sanger sequencing reactions on different...Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK or DLYDDDDK ...