...Background Förster resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred...fluorophore to an acceptor. Since the transfer of energy does not occur by emission of a photon, the acceptor...pairs in FRET experiments. The efficiency of the energy transfer is measured using fluorescence microscopy...

...making Integrase deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and...N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag... with 2nd and 3rd generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for...resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven...

...Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. 2017. Enhanced...PMID: 27984730 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...Science . 343(6166):80-4. PMID: 24336569 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost...

...activation plasmids and screening library CRISPR/Cas9 Synergistic Activation Mediator (SAM) is an engineered protein... editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature . ...aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

...production, all antibody preps are titered and undergo stringent quality control by Addgene or a trusted...Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The...distributed. Quality Control Each lot of antibody undergoes testing, either in-house or through outside labs...

...puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA Negative control vector... arrayed viral high-content screen. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, Piqani...An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD. RNA. 2003. 9(4):493-501. PubMed...

... Next-generation GRAB sensors for monitoring dopaminergic activity in vivo. Coexpressing red GRABDA with...calcium indicator GCaMP6s allows tracking of dopaminergic signaling and neuronal activity in distinct ...resource was created with support from the Chan Zuckerberg Initiative (Link opens in a new window) ....

...3rd 10 Varies Berger Lab Human Paralog Knockout Library (pgPEN) 171172 Knockout Human Berger 3rd 8-16 33,170...

...delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786. PMID:30295607...Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range..., Fuzik J, Fürth D, Fenno LE, Ramakrishnan C, Silberberg G, Deisseroth K, Carlén M, Meletis K. 2019. A...

... strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core has 100% seq conservation...BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-...

...visit iGEM: Silver Standard (RFC 23) Back to Top Freiberg Standard (RFC 25) Prefix: GAATTC GCGGCCGC T TCTAGA...NgoMIV, AgeI, SpeI, PstI For more info, visit iGEM: Freiberg Standard (RFC 25) Back to Top...

...ancestral capsid Anc80L65 with endogenous AAP Vandenberghe 68837 pAnc80L65 Anc80L65 AAV packaging plasmid...expressing Rep2 and ancestral capsid Anc80L65 Vandenberghe 78504 pGG-B1 B1 AAV packaging plasmid, expressing...

...standard viral production , these viral vectors undergo quality control, including AAV titration by ddPCR...chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2(Y444F) When...

...-EBD - Estradiol-dependent control Gal1 Yeast Gartenberg 50797 pUAS-Cre Cre UAS/Hsp70 Mammalian Cepko ...plasmids, reporter genes indicate which cells have undergone Cre recombination. These plasmids can be useful...Proc Natl Acad Sci. Jul;85(14):5166-70. PubMed . Sternberg N, Hamilton D. 1981. Bacteriophage P1 Site-Specific...

...dJ579N16.4 ADRB1 adrenergic, beta-1-, receptor ADRB1R, B1AR, BETA1AR, RHR ADRB2 adrenergic, beta-2-, receptor...CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPR30, LERGU, LERGU2, LyGPR, MGC99678 GPHA2 glycoprotein hormone ...activates the T cell, whereas T cell tolerance or anergy are induced when the TCR is engaged without co-...LHRHR, LSH-R LIF leukemia inhibitory factor (cholinergic differentiation factor) CDF, DIA, HILDA LIFR ...

...sequences are now available and more are currently undergoing quality control analysis in our lab and will ...2020.05.04.20091231 AapCas12b plasmid from the Abudayyeh-Gootenberg lab. A one-enzyme RT-qPCR assay for SARS-CoV-...

...IgG2a 206555 Anti-Alpha-2C adrenergic receptor [N330A/80R] Alpha-2C adrenergic receptor Human Mouse IgG2a...monoclonal antibodies are produced in-house and undergo application-specific validation and quality control...

...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...

...disease (AD) by generating validated resources for emerging targets in AD and creating high-quality, well-...

...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...