...may also like... Julian Rayner Lab plasmids Gavin Wright Lab plasmids This is a collection of plasmids that...

...separate proteins based on size. Whether higher molecular weight complexes are detected will depend on the...series described a practical approach to selecting a bright fluorescent protein and a photostable fluorescent... have a natural affinity and a tendency to form higher order aggregates. This property can be tolerated...purified fluorescent proteins to form homodimers or higher order oligomers can be analyzed by several techniques...essential to verify a number of key properties (brightness, photostability, oligomerization) of a number...will be using. If you do a head-to-head comparison right, it will be valuable information for the scientific...

...molecule. A higher brightness increases the detected signal and therefore high brightness is an important...EGFP). The higher the number, the higher the theoretical brightness. The theoretical brightness often appears...are (i) brightness, (ii) photostability and (iii) aggregation tendency. Brightness Brightness refers ...practical brightness is a better criterion than theoretical brightness for selecting the brightest fluorescent...and the sample. Practical brightness A protein with a high intrinsic brightness that does not fold well ...how the brightness is best defined or determined. Here, we will explain how practical brightness is determined...protein. Theoretical brightness Two key properties of fluorophores that determine brightness are the extent ...

...mutagenesis for the brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission...mWasabi, a bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter than EGFP...-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein ...protein at the time. It is 1.5 to 3 times brighter than the most commonly used GFPs and YFPs. Its excitation...changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein The team that developed...Hui-wang, et al. "Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent...PMCID: PMC2292683. Shaner, Nathan C., et al. "A bright monomeric green fluorescent protein derived from...

...ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for designing...way to design GGA overhang sets, particularly for high-complexity reactions? The researchers over at New...otherwise! They suspected a data-driven approach might allow for an increase in the complexity. In 2018...need to be strictly followed in order to generate high-fidelity reactions. (Need a refresher? The five ...100% GC.) In fact, the NEB® team’s data showed that high-fidelity reactions could be done using overhang ...sets that broke rules 3—5. Fidelity, it turns out, might depend more on the individual sequence(s) than on...build out three tools to help researchers design high-fidelity Golden Gate Assemblies and overhang sets...

...Investigators, an open-access journal that enables high school students to publish peer-reviewed scientific...behind the journal and hear from a fantastic team of high school students who recently submitted to the journal...Journal of Emerging Investigators. Chapter 2 - Getting high school students and teachers interested in JEI (...mentorship programs. Chapter 6 and Outro - Interview with high school student researchers Arooba Ahmed, Jiachen...

...with the flick of a light switch. Neurons are not typically persuaded to fire when light is shined on them...expression of light-gated ion channels such as channelrhodopsins (ChRs) makes them light-responsive. When...mm3). Current channelrhodopsins also need high-intensity light for activation which requires implantation... no blue light, the channelrhodopsin remains inactive and neurons don't fire. With blue light, ions flow...are highly constrained and poorly understood. It’s hard to design new channelrhodopsins with high-throughput...requires higher expression levels in order to activate a neuron. ChRgers, however, have a higher conductance...the light was turned off.  Together these two experiments determined that ChRger2’s improved light sensitivity...

...dark state and highest fold induction at 31X. By using a slit pattern during blue light exposure (470nm...proteins N713-pMag and nMagHigh1-C714, this new system had both low background and high fold-induction of ...reversibly activated by turning blue light on and off. As one might expect from the modular nature of Cas9...fused with high-affinity interacting proteins Coh2 and DocS, respectively. The use of far-red light is of ...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed and...tissues. Additionally, high intensity exposure can cause DNA damage. Far-red light, however, can penetrate...protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity Anti-CRISPR proteins are a highly diverse ...

...framework encoded in these plasmids robustly yields highly active artificial transcription factors ( Maeder...

...and Cpf1, which require that each DNA target have high sequence identity to the spacer sequence and contain...

...Erythrocyte Receptors You may also like... Gavin Wright Lab plasmids This is a collection plasmids for ...

...CRISPR-based purification methods are more straightforward and enable direct identification of molecules...

...resulting in frameshifts. If a repair template with high homology to the DNA surrounding the DSB is introduced...

...fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular signals...

...services to you, we must ensure that you have the right to use the original plasmid. Distribution of the...

...for Protein Innovation Collection : Antibodies to highly conserved proteins and other difficult-to-target...

... target various genes or genomic regions. It is highly recommended that you determine if your cell type...

...Plasmodium falciparum You may also like... Gavin Wright Lab plasmids This is a collection of plasmids that...

...FLASH (Fast Ligation-based Automatable Solid-phase High-throughput) assembly method (Reyon and Tsai et al...

...researchers, we wondered how we could make the insights gained during this time more visible and thereby... ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video to learn ... ground again and again as they don’t save the insights they glean from a reading session. To remember...hunting through a whole folder of papers to find the right experiment! The ReFigure extension also tells readers...ReFigure making it easy for others to discover your insights on a topic. Beyond collating other’s data, you...include your own data in a ReFigure. For example, you might include a confirmatory image to show that a previously...previously used reagent performs well, or you might publish a replication experiment that you conducted ...