Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Showing: 1 - 20 of 213 results
  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...practical due to the low intensity of luminescent proteins. To make luminescent imaging a reality, Addgene depositor...with Venus. This luminescent technology is based on the principle of bioluminescence resonance energy ... a few drawbacks associated with luminescent imaging: A luminescent substrate is required for imaging...Researchers have long been interested in using luminescence to get around these issues, but this solution...fusion proteins. Solving the problems of low luminescence By itself, Renilla luciferase has a low quantum... hasn’t stopped some scientists from using chemiluminescent proteins for imaging, but it has precluded...orange Nano-lanterns and showed that these three luminescent proteins can be used together in cells. As Nano-lanterns...
  2. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...eScience Labs as part of their curriculum by either taking existing curriculum and customizing it for schools...the entire curriculum development team at eScience Labs. She still gets to write curriculum, but has taken...Caitlin’s take on her job as an SME: “I write curriculum for students taking science courses online, which...student the best possible experience.” Managing curriculum development About a year after Caitlin was hired...
  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Endoplasmic Reticulum KDEL sequence mNeonGreen Dorus Gadella 137805 ER-mScarletI Endoplasmic Reticulum KDEL ...Endoplasmic Reticulum ER retention signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention...Endoplasmic Reticulum BiP ER signal sequence BFP Gia Voeltz 61807 Rtn4a-GFP Endoplasmic Reticulum Rtn4a AcGFP...Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic Reticulum Sec61 BFP Gia...Endoplasmic Reticulum CytERM mScarlet-I Dorus Gadella 85067 pCytERM_mScarlet-H_N1 Endoplasmic Reticulum CytERM...Endoplasmic Reticulum CytERM mScarlet Dorus Gadella 158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin...Proteins are first translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in...
  4. Luciferase Plasmids

    Type
    Collection
    ... of experimental systems. LumiLuc and LumiScarlet : LumiLuc and LumiScarlet are ATP-independent luciferases...Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights...Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging... cells must be lysed in order to monitor the luminescence, making time-course experiments impossible. ...time course experiments possible, but its short luminescence half-life makes it too dim for many experimental...Highlights of Luciferase Tools in our Collection Bioluminescence Resonance Energy Transfer (BRET) Nano-lanterns...-based C o- I mmunoprecipitation) plasmids : Luminescence-based protein-protein screening method for the...
  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...AICSDP-41 mEGFP SERCA2 Sarcoplasmic reticulum/endoplasmic reticulum 114406 SMC1A-mEGFP AICSDP-57 mEGFP ...SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin Nucleolus...tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell . 28(21):2854...
  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Expression mPlum 590 649 4 4.5 100 min Monomer pQC mPlum IX - Mammalian Expression mPlum-N1 - Mammalian...Mammalian Expression mPlum-C1 - Mammalian Expression mPlum-pBAD - Bacterial Expression mRaspberry 598 625 13...
  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...2 O to final volume of 50 μl. This reaction may be scaled down to a smaller final volume if necessary....contains 50 μl cell culture media per well for a final volume of 150 μl. This facilitates screening by allowing...Add an additional 100 μl to each well for a total volume of 200 μl. Wait 24 - 72 hr to allow cells to grow...
  8. Plasmids for Optogenetics Research

    Type
    Collection
    ....1-aDTX-lumitoxin Lumitoxin Mammalian mCherry Boyden 51689 pcDNA3.1-CONK1-lumitoxin Lumitoxin Mammalian...Mammalian mCherry Boyden 51691 pcDNA3.1-DTXK-lumitoxin Lumitoxin Mammalian mCherry Boyden 53095 PA-DAD-wt LOV...
  9. Serotype Testing AAV

    Type
    Collection
    ...for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 37825-AAV1.T 20 µL $100 Add to Cart...for each catalog ID below). Serotype Catalog ID Volume Price* AAV1 50465-AAV1.T 20 µL $100 Add to Cart...
  10. AAV Made on Request

    Type
    Collection
    ...and feedback on this service. AAV Service Details Volume 1-1.5 mL in 100 µL aliquots Minimum Titer 5E12 ...AAV serotype. Please note that depending on the volume of requests, we may not be able to accept all orders...
  11. Plant Plasmids and Resources

    Type
    Collection
    ...general and species-specific gene expression (see the column ‘promoter’ in the tables for species-specific tools...highlights plasmids that feature fluorescent or luminescent proteins for expression in plants. You can search...
  12. COVID-19 Resources

    Type
    Collection
    ...in solution that are suitable for in vitro use. Volume and minimum titers are listed on each item's material...nonprofits, and this is denoted in the Industry column below. For more information on ordering from a ...
  13. Optogenetics Guide

    Type
    Guide
    ...sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2 photoswitch. Illumination causes...expression of the opsin and the area that is being illuminated. There are several different ways to control ...2006. Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci...
  14. Molecular Biology Reference

    Type
    Guide
    ...acquire NGS but one of the most commonly used is the Illumina NGS platform. This is the platform used by Addgene...sequencing partner, seqWell . The actual process of Illumina NGS is not that different from Sanger sequencing...modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented into...regular base and thus do not halt the reaction. Illumina NGS, therefore does not require any “normal” bases...
  15. Fluorescent Protein Guide

    Type
    Collection
    ...Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I...
  16. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...on this service. DNA Service Details Amount 2 µg Volume 20 µl Concentration 100 ng/µl (± 5ng/µl) Buffer...
  17. Plasmid Collections

    Type
    Collection
    ... Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics...
  18. Lentiviral Prep Service

    Type
    Collection
    ...in solution that are suitable for in vitro use. Volume and minimum titers are listed on each item's material...
Showing: 1 - 20 of 213 results