... heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be ...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802...heterodimeric FokI domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 ...

... of detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like...imaging has numerous advantages over other imaging techniques, including that it is easy to implement due to...FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics in living cells. The fluorescent...

...trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also have an older system using two...express up to three sgRNAs (Swiech et al., Nature Biotechnology 2014). Full references are below. Available ...mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements...Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;31(9):827-32. doi: 10.1038/nbt.2647...Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055...

...bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini... expressed in vivo . Plasmid ID Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent...Reporter Plasmids Reporter plasmids can be used to detect events and molecules in and around a bacterium....small molecule, the reporter plasmid can be used to detect the presence of that small molecule and sometimes...) in the plasmid. Other similar reporters for detecting maltose can be found here . pAra Series Various...

...assembled a list of FAQs about using the lab's CRISPR technology....assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn ...addition to mutations in the spacer itself, will protect the HR template from the Cas9. Again, if it's within...of the Cong, et. al, 2013 Science paper). If you detect positive HR, isolate single-cell colonies, grow...

...the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify three different sites in the EGFP reporter... reporter gene (Fu et al., Nat Biotechnol. 2013). The expression plasmids encoding the guide RNAs ( gRNAs...Additional details can be found in Fu et al., Nat Biotechnol. 2013 and users are encouraged to post questions...

...( RGN ) technology to modify four different endogenous human genes (Fu et al., Nat Biotechnol. 2013). ...Additional details can be found in Fu et al., Nat Biotechnol. 2013 and users are encouraged to post questions...

...of genomic deletions. In addition, we describe techniques for deletion, identification, and characterization...bp from the predicted cleavage site to ensure detection would not be impacted by a small indel at the ...in Molecular Biology. 2001. PubMed . MicroRNA detection in prostate tumors by quantitative real-time PCR...PubMed . Western blotting: sample preparation to detection. Eslami A, Lujan J. J. Vis. Exp . 2010...

...the use CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology to modify nine different sites in various endogenous...endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The expression plasmids encoding the ...details can be found in Hwang & Fu et al., Nat Biotechnol. 2013 and users are encouraged to post questions...

...practice CRISPR-Cas/RNA-Guided Nuclease ( RGN ) technology in zebrafish. The Joung Lab has demonstrated ...endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The Cas9 expression vector listed below...zebrafish can be found in Hwang & Fu et al., Nat Biotechnol. 2013. The guide RNA ( gRNA ) expression vector...

...England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental Information Background ...fixed cells. The plasmids associated with this technology are available at Addgene for distribution to ...comparison to GFP, please refer to this table. Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...

...proteins, visualize a protein under a microscope, or detect when the protein is present in a sample--just to...Vector system for expression, dimerisation and detection of recombinant antibody fragments in the form ...and utilisation of recombinant antibodies. BMC Biotechnol. 2006 Dec 7;6:46. John McCafferty CRISPR system...

...spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation...CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR Resources for a full selection...initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement ...

...CRISPR Guide for a general introduction to CRISPR technology or the zebrafish CRISPR resources for a full ...zebrafish resources and developing new lines and technology. European Zebrafish Resource Center (EZRC) - ...- A repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines ...

...2009 (Hoshino & Fujii), the locus-specific ChIP technologies enable us to purify specific genomic regions...interacting with those regions. Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation...DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins. ...

...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction...48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b vectors were designed...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

...Krueger C, Pfleiderer K, Hillen W, Berens C. Biotechniques . 2004 Oct;37(4):546, 548, 550. PubMed . Optimization... R, Heinz N, Hampf M, Bujard H, Gossen M. BMC Biotechnol. 2010 Nov 24;10:81. doi: 10.1186/1472-6750-10...10-81. PubMed . Tet systems : Science and Technology Do you have suggestions for other plasmids that should...

... Stem Cell Research page. CRISPR Tools CRISPR technology is a promising therapeutic approach for precise...CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR resources for a full ...Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in a new window...

...research and development of engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas... nuclease fusions Related Genome Engineering Technologies TALEN Engineering Reagents CRISPR Plasmids...

...given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of...