...differentiate into many different cell types in the body during development. Unlike other cell types, each stem...and Klf4) that are known to maintain pluripotency during embryonic development. This “reprogramming” of ...plasmids Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell...separate plasmids Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. ...

...Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration - Periklis Pantazis...combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous single-cell profiling of lineages...zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R package for analyzing ...

...Improved ATP FRET Sensor For Yeast Shows Heterogeneity During Nutrient Transitions. ACS Sens. 2020 Mar 27;5(3...-0. Yulong Li ATP/ADP Monitor the ATP:ADP ratio during live-cell imaging (Perceval) A genetically encoded...Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. J Neurosci...Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy. Mol Cell. 2019 Apr 18;74(2...regulation of the mitochondrial proton gradient during cytosolic calcium elevations. J Biol Chem. 2011...

...of shipment. If any discrepancies are identified during this analysis, you will be notified by email. If...thorough shipping documents and following import procedure, it is the responsibility of the importer to pay...

...the pTT3 plasmid backbone which is described by Durocher et al. Nucleic Acids Research 2002 Jan 15;30(2... Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner JC and Wright GJ Nature 2011 Nov 9...

...a pathogen and is the dominant defense strategy during the first several days following exposure to a ...through the B cell receptor (BCR). The strength and duration of signaling controls the fate of B cells and ...communicate with each other across long distances. During infection and inflammation, the different cytokines...macrophages and sense conserved microbial molecules during an infection. When TLRs sense a microbial pathogen...

... TBK1 Activation to Promote Mitophagy. Heo JM, Ordureau A, Paulo JA, Rinehart J, Harper JW. Molecular ... described in: Enhanced phosphoserine insertion during Escherichia coli protein synthesis via partial ...

... one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production. Bhadra S, et al. bioRxiv...access to articles related to SARS-CoV-2 research during the current outbreak: Cell Press Coronavirus Resource...

...recombinases are present, the introns are excised during mRNA processing, producing a functional molecular...Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window) with general principles...

...modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically or genetically ...cytidine to uridine, which is read as thymidine during subsequent DNA repair, creating a C to T change...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...with a 24 bp sequence, to simplify identification during screening. Library ID PI Description MORF library...

...proteoglycans crucial for regulating cell signaling during development. The IPI collection includes anti-glypicans...

...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally...

... (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the Cas9 nickase...

...specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV Guide for more information...

...sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus ...

...AAV Production 6–8 weeks Quality is our priority. During this time, we amplify DNA, produce the prep, and...

...amplify a portion of the intended lentiviral insert. During this cultivation, cells are routinely checked for...

...1, which may require additional lab biosafety procedures. Additionally, because this virus integrates ...

...mid-sixties, or late-onset, where symptoms appear during or after a person's mid-sixties. The early-onset...