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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 33 results for: abo.5

Showing: 1 - 20 of 33 results

  1. Optogenetics AAV Preps

    Type
    Collection
    ...1, 2, 5, 9 Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn ChR2/H134R EYFP Constitutive 1, 2, 5, 9, rg...Constitutive 1, 5, rg* Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn ChrimsonR tdTomato Constitutive 1, 5, 9 Boyden... dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Boyden...Constitutive 5, 8, rg* Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Jaws GFP Cre dependent 1, 5, rg* ...Constitutive 1, 5, rg* Yizhar 125713 AAV-hSyn1-SIO-eOPN3-mScarlet-WPRE Syn eOPN3 mScarlet Cre dependent 1, 5 Yizhar...Constitutive 1, 5 Yizhar 198511 pAAV_hSyn-DIO-PdCO-mScarlet-WPRE Syn PdCO mScarlet Cre dependent 1, 5 Yizhar 198513... 1, 5 Yizhar 198516 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE EF1a PdCO mScarlet Cre dependent 1, 5 Yizhar...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...(A & B) Knockout Mouse X.S. Liu 3rd 5 5 10 49,252 Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation...Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093 Activation Mouse Weissman 3rd 5 10 107,105...Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092 Inhibition Mouse Weissman 3rd 5 10 107,415...Weissman 3rd 5 Varies hCRISPRi-v2 subpooled libraries 83971 — 83977 Inhibition Human Weissman 3rd 5 Varies ...Weissman 3rd 5 Varies mCRISPRi-v2 subpooled libraries 83989 — 83995 Inhibition Mouse Weissman 3rd 5 Varies ...Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide library v2 67988 Knockout Mouse Yusa 3rd 5 90,230 Mouse... Library v2 131625 Inhibition E. coli Bikard N/A ∼5 21,417 Bison sgRNA Library 169942 Knockout Human Ebert...

  3. Chemogenetics AAV Preps

    Type
    Collection
    ...Cre-dependent 5, 9 Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson...EGFP none 5 Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion none 2, 5 Richie 121539...mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion none 5 Roth 50479...) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362 pAAV-hSyn-DIO-hM4D(Gi...) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-...mCherry hM3D(Gq) - Activation mCherry fusion none 1, 2, 5, 8, 9, rg* Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry hM4D... hM4D(Gi) - Inhibition mCherry fusion none 1, 2, 5, 8, 9, rg* Roth 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-...

  4. Recombinases AAV Preps

    Type
    Collection
    ...Cre.SV40 CamKII none 1, 5, 9, rg* Wilson 105537 pENN.AAV.CMVs.Pl.Cre.rBG CMV none 1, 2, 5, 8, 9, rh10 Wilson...pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Carmichael 107788 AAV.rTH.PI.Cre.SV40...EBFP-Cre Syn EBFP 5 Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB...105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a...Deisseroth 121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG... 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn none 1, 2, 5, 8, 9, rg* Wilson 105555 pENN.AAV.hSyn.Cre.hGH Syn...107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB Larsen 107787 AAV.TBG.PI.Cre.rBGe...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3 HTR3B 5-hydroxytryptamine...heavy diversity 5-24 (non-functional) IGHD524 IGHD5-5 immunoglobulin heavy diversity 5-5 DK4, IGHD55 IGHD6...:14099, K5-5, MGC88293 CCR5 chemokine (C-C motif) receptor 5 CC-CKR-5, CCCKR5, CD195, CKR-5, CKR5, CMKBR5...receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor 3, family member C - HTR3D 5-hydroxytryptamine... interleukin 5 (colony-stimulating factor, eosinophil) EDF, IL-5, TRF IL5RA interleukin 5 receptor, alpha...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... four Venus fluorescent proteins connected via a 5, 5 and 6 amino acid linker, respectively VVVVV Anisotropy...Plasmid FRET Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus ... acid linker C5A Cerulean attached to Amber via a 5 amino acid linker C17A Cerulean attached to Amber ...of two Venus fluorescent proteins connected via a 5 amino acid linker V17V Anisotropy/brightness standard... three Venus fluorescent proteins connected via a 5 and 6 amino acid linker, respectively VVVV Anisotropy... of five Venus fluorescent proteins connected via 5 amino acid linkers VVVVVV Anisotropy/brightness standard...consisting of six Venus fluorescent proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...parameters: 37 °C for 30 min; 95 °C for 5 min and then ramp down to 25 °C at 5 °C/min. Dilute oligos 1:10 in ddH...following parameters: Cycles 1-20 (37 °C for 5 min, 20 °C for 5 min); Cycle 21 (80 °C for 20 min). These ...enzyme (5,000 U/ml), 5.0 μl ATP (10 mM), 0.25 μl (5 μg) BSA (20 mg/ml), 0.375 μl (750 U) T4 DNA ligase... solution and add to electroporation cuvette. Add 5 μg of each CRISPR/Cas9 construct (10 μg total). Add...construct. Electroporate cells with 250 volts for 5 msec in a 2 mm cuvette using an electroporation system...population exposed to sgRNA-A and sgRNA-B (see step 5 ). The purpose of the following PCR is to validate...multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and remove supernatant by flicking the PCR plate...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...Insertion of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5' promoter.... Jorge Ferrer 16539 pBV-Luc Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0...normalization William Kaelin 71248 pXPG Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase...Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream of Firefly luciferase...Mikko Taipale 106253 pLS-mP-Luc Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv...Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv...pGL3-TK-5UTR-BsmBI-Luciferase Firefly Insertion of 5' promoter/enhancer regions. Blue/white screening can...

  9. CRISPR Guide

    Type
    Collection
    ...bacterium (LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...will continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches...inhibit target cleavage, while mismatches toward the 5′ end distal to the PAM often permit target cleavage...from naturally occurring cytidine deaminases. Figure 5: Overview of base editing More recently, researchers...transcription. The edited DNA strand displaces the unedited 5’ flap, and the resulting heteroduplex is resolved ... repair using the edited strand as a template PE4/5 - improved PE2/PE3 to inhibit mismatch repair to promote...

  10. Neurodegeneration Research Collection

    Type
    Collection
    ...and breathing, and often results in death within 3-5 years from symptom onset. Disease mechanisms are still...having no prior family history. A small percentage (5-10%) are familial ALS cases having at least one other...knockout . Maimaitili et al. Nat Commun. 2023 Dec 5. See More Antibodies Find information on Addgene's...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

  11. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Low Adenoviral Transient Double-stranded linear DNA 5–36 kb 105 nm Dividing/Non-dividing No High *Recombinant...Page Viral Vectors 101 eBook Content last reviewed: 5 August 2025...optimized for both safety and efficiency. Learn more about the different types of viral vectors and find viral...

  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. Teresa Alonso , Javier García-Sancho Calcium Genetically... calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. Amy Palmer , Roger Tsien Calcium Turquoise... in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent Mg2+ sensor...influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10):e257. Wolf Frommer Return to Top Neurotransmitters...2023.05.27.542566. Yulong Li Serotonin Serotonin (5-HT) sensor GRAB_5-HT1.0 for viral expression in neurons...measuring serotonin dynamics. Nat Neurosci. 2021 Apr 5. Yulong Li , J. Julius Zhu Serotonin Serotonin sensor...S0092-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery...

  13. Ras Pathway

    Type
    Collection
    ...Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251/wjgo.v5.i5.97. PubMed PMID... Frederick National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research...

  14. AAV Viral Preps

    Type
    Collection
    ... vectors for trial purposes in serotypes AAV1, 2, 5, 6, 8, 9, 11, and retrograde Retrograde AAV To target...it will be listed on the Function section pages above. You can also do a search of viral preps and filter...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...20R] SUR2B Rat Mouse IgG2a 114481 Anti-QKI-5 [N195A/16R] QKI-5 Human Mouse IgG2a 114482 Anti-Synaptotagmin... IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a 128612 Anti-Kv2.1... IgG2a 177570 Anti-mGluR1/5 (Group I) glutamate receptor [N75/33R] mGluR1/5 (Group I) glutamate receptor...Mouse IgG2a 182119 Anti-mGluR1/5 (Group I) glutamate receptor [N75/3R] mGluR1/5 (Group I) glutamate receptor...Mouse IgG2a 206550 Anti-Synaptotagmin-5 [N274/8R] Synaptotagmin-5 Mouse Mouse IgG2a 206552 Anti-Histone... TRIP8b (exon 1a-5) (Mus musculus) recombinant monoclonal antibody. TRIP8b (exon 1a-5) Mouse Mouse IgG2a...190517 Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse...

  16. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

  17. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...iSPI_pSer_Subpool#6 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188529 iSPI_pSer_Subpool#4 Pooled Library...1371–1375. https://doi.org/10.1038/s41592-022-01638-5. PMID 36280721 (Link opens in a new window) rEcoli...collection page Read about Using Phosphoserine to Study Protein Phosphorylation Read about GCE4All: Making ...Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

  18. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually ...Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The ...

  19. Plan Your Experiment

    Type
    Collection
    ... regions, 5’ exons, or an exon known to code for an essential protein domain. Targeting a 5’ exon reduces...CRISPR/Cas9-based genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 PMID: 26066060...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...controls when working out a new protocol. Read more about how to design your gRNA . Synthesize and Clone Desired... other downstream methods. For more information about viral vectors and their production, see our viral...and Proteins In addition to the methods described above, you can also directly deliver mature mRNA or purified... approach. Similar to the mRNA method described above, this method is also transient, as the RNPs will...

  20. Brain Initiative Collection

    Type
    Collection
    ...Fishell 83896-AAV1 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAV9 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain... 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons Yulong Li 140553...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...
Showing: 1 - 20 of 33 results