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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 100 results for: PLE;

Showing: 31 - 60 of 100 results

  1. Rett Syndrome

    Type
    Collection
    ...methylated DNA and the recruitment of the NCoR complex for proper nervous system function. While there...RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines are...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page...precise editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources can be found below...cell line repository (Link opens in a new window) Sampled - one of the world's largest biorepositories (Link...your plasmids! To get started with your deposit, please click here ....

  2. Antibody Production

    Type
    Collection
    ...total protein content of the sample is determined. Only antibody samples with >90% purity are distributed... webpage. To learn more about these antibodies, please visit the Institute for Protein Innovation Antibody...using a NanoDrop spectrophotometer. If needed, the sample is adjusted by diluting or concentrating to a final...Coomassie staining. For Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside...specific QC experiments were performed on your lot, please email [email protected] . Immunocytochemistry (ICC...

  3. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...imaging techniques, including that it is easy to implement due to the simplicity of gRNA design, programmable...for different genomic loci, capable of detecting multiple genomic loci, and compatible with live cell imaging...copies of labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A non-repetitive...genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting...

  4. AAV for Neuronal Tracing

    Type
    Collection
    ...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement deletion-mutant rabies virus AAV1 Wickersham ...lacks the ability to infect neighboring cells. For example, G-deleted rabies lacks the viral glycoprotein...infect any neighboring cells. Thus, through complementation, G-deleted rabies virus can spread to monosynaptically...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  5. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...far more complex than using CRISPR to modify a single genomic locus. Before ordering, please review the...consist of thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening...Root 2nd 2 40,710 Inzolia Human CRISPR/Cas12a Multiplex Knockout 209551 209552 Knockout Human Doench 3rd...117725 Knockout Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ...

  6. Plant Plasmids and Resources

    Type
    Collection
    ... seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose...CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants - Yao-Guang...

  7. Ras Pathway

    Type
    Collection
    ... browse the gene list below. For proteins with multiple subunits or isoforms, individual links to each...available plasmids for the gene. For components with multiple subunits or isoforms, individual links to each...EXOC2 EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast... cis/trans isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ...movement. More than ten million people worldwide are living with PD, mostly people over the age of 60. There...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual...variation and lifestyle or environmental factors. For example, variations of Apolipoprotein E (APOE), such as...collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link opens in...

  9. Cre-lox system

    Type
    Collection
    ...PGK Retroviral Lowe 33344 pLEW100cre-EP1-6G Cre T. brucei Cross 33345 pLEW100cre-EP1 Cre Trypanosoma brucei...blog post. Based on these Cre-lox recombination principles, scientists have developed constructs to activate...pSkipFlox split Cre Pfcrt P. falxiparum Spielmann 86641 pLenti-hSynapsin-Cre-WPRE Cre hSyn Lentiviral Wang 86794...donor/transfer vector Cbh AAV Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC...in the indelible marking of mouse neurons with multiple distinct colors Mammalian Sanes See article Multibow... to facilitate Cas9 mediated genome editing in multiple tissues. FLP/FRT: Alternative recombinase system...

  10. Validated gRNA Sequences

    Type
    Collection
    ...cut S. pyogenes 26028531 Huangfu OCT4 H. sapiens multiple, see article 69537 activate S. pyogenes 26352799... pyogenes 25336740 Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325...23817069 Calarco Kras, p53, and Lkb1 M. musculus multiple, see article 60224 cut S. pyogenes 25263330 Zhang.... pyogenes 23929340 Kamoun PDS1 N. benthamiana multiple, see article 49772 cut S. pyogenes 24112467 Kamoun...AGCACTGTTGCCGGCCGAGG 79006 cut S. pyogenes 26944679 Shaw Please refer to the primary article to confirm details...add validated gRNA sequences to our datatable, please download the following spreadsheet, fill out as...

  11. Caltech Systemic Capsids

    Type
    Collection
    ...control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible) in vivo...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER... serotype will work in your mouse line, see Supplementary Table 3 (Link opens in a new window) . Browse...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...

  12. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ... 12 GC/mL for the AAV2 serotype, as titered by droplet digital PCR (ddPCR) . In some cases, we may expect... sequence verification, may not yet have been completed at the time of shipment. If any discrepancies ...for any additional customs fees or taxes, and to complete any additional customs documentation that might...for the import of biological research materials. Please be aware that additional customs charges may be...

  13. Antibody Plasmid Collection

    Type
    Collection
    ...microscope, or detect when the protein is present in a sample--just to name a few. Traditional sources of antibodies...individual clone targeting a single antigen. An example of R-mAbs is the NeuroMab collection - monoclonal...single-domain antibodies (also known as nanobodies). For example, scientists often use plasmids encoding scFv fragments...recombinant antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation...

  14. Lentivirus Plasmids

    Type
    Collection
    ...particle (a virion) are generally divided among multiple plasmids. Some plasmids (typically called packaging...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search... See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with... See article for more variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection...

  15. COVID-19 Resources

    Type
    Collection
    ...virus Mammalian constructs Due to the size and complexity of the collection, all available Ginkgo SARS-...information on ordering from a for-profit entity, please see our Plasmid Distribution to Industry page. ...top General Plasmid Tools SHERLOCK and DETECTR - Simple, portable, and inexpensive platforms to reliably...Collections of COVID-19 related articles spanning multiple publishers can be found here: ASM COVID-19 Research...

  16. Depositor Collections

    Type
    Collection
    ...Dehydrogenase CUREs Community (MCC) Collection Pleiades Promoter Project: mini-promoters to drive selective...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral Vector System - Dankort...

  17. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense...instead recruiting the transposase (comprised of multiple components) to target locations in the genome ...

  18. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex, comprised of a combination of ...difficulties in getting all components of the Cascade complex to express equally. To combat this, some CRISPR-Cas3...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's collection: HIV-based lentiviral...and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for measuring...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...

  20. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Bacterial Expression mApple 568 592 37 6.5 30 min Monomer mApple-N1 - Mammalian Expression mApple-C1 - Mammalian...Mammalian Expression mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-...

  21. Microbiology Resources

    Type
    Collection
    ...Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye Lab Vaccinia... in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas...

  22. Lentiviral Prep Service

    Type
    Collection
    ...Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but...

  23. Viral Vectors

    Type
    Collection
    ... wide array of genetic functions in cells. For example, if there is a protein thought to be associated..., are suited for specific research goals . For example, adeno-associated virus (AAV) is typically preferred...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...

  24. Tetracycline Inducible Expression

    Type
    Collection
    ...transcriptional activation domain from HSV (herpes simplex virus). In the absence of tetracycline, the tetR...Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains an insert (MAT2A) which... Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral reverse tetracycline-controlled...

  25. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Plasmids are available for all the items listed below. Please let us know (Link opens in a new window) what you...you would like to see available in viral format. Please note this does not guarantee viral service, but...pENN.AAV.CB7.CI.TurboRFP.WPRE.RBG James M. Wilson Please contact [email protected] with any questions. Return...

  26. Genetic Code Expansion

    Type
    Collection
    ...can bind to the mRNA (hence, the anticodon is complementary to the codon), the amino acid from the tRNA ...typically expressed from a single plasmid, with multiple copies of the tRNA. Applications By making small... and all questions you have while designing, implementing, or troubleshooting GCE experiments in your ...

  27. AAV Viral Preps

    Type
    Collection
    ...Rabies Virus-Based Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes...

  28. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids for neuronal excitation and inhibition. For example, search for "ChR mCherry", "Cre inhibition", "Boyden...to control a protein or cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other...

  29. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...be used with any CRISPR nuclease and function. Please see the publication or plasmid information page...

  30. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Our production process and quality control, with examples of quality control data. Help Center Articles ...will also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that...
Showing: 31 - 60 of 100 results