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We narrowed to 137 results for: Ide

Showing: 21 - 40 of 137 results

  1. TALEN Guide

    Type
    Collection
    ... TALENs TALEN Guide Addgene's Guide to TALEN Technologies...Scientists have identified a large number of zinc fingers that recognize various nucleotide triplets. With...Guide to TALEN technology and using TALEN plasmids TALENs...disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ... and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...pAAV-CaMKIIa-Flpo None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... Cre Yes Dual recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ... recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral ...

  3. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules associated... are more straightforward and enable direct identification of molecules associated with a genomic region...

  4. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    .... Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene...Gene/Insert PI Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require...require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence...degradation seen in bacteria. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

  5. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Special Collections Fluorescent Protein Guide Fluorescent Protein Plasmids & Resources You May... For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent...variety of fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent...fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular... actuators or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically engineered...Localization Determine where your protein of interest resides by using a well-characterized fluorescent fusion...

  6. Caltech Systemic Capsids

    Type
    Collection
    ...opens in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306...plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-...plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP... (Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-...

  7. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...and plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter... Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...e.g. "RCaMP", "caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen peroxide Monitoring the ...Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular hydrogen peroxide concentration...

  9. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...glycine- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting...Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources: Empty ...proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected...and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected...connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected by... by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected by 5 GGSGGS...GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS repeats...

  10. Viral Vector Guides and Plasmids

    Type
    Collection
    ...viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . Resources Addgene’s ...viral vector guides and plasmids available at Addgene and other related resources. Science... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...find viral production and transfer plasmids for a wide array of research purposes. Interested in Addgene's...permanently integrated into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors...

  11. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6...Available as a Viral Vector Packaging Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1...this table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This...based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral... virus. Instead, specific gene products can be provided by helper plasmids (e.g., pHelper) and specific...HEK293 cells) during AAV production. Read our AAV Guide for more information about: AAV components Common...

  12. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Return to top Other Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter..., Dre, etc.) or target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene...selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled...empty backbones or reporters . Experimental Considerations Recombinase Recognition Sites Each enzyme functions...

  13. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...

  15. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Collections Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In Vivo Imaging More Fluorescent...photoactivatable fluorescent empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers of cytoskeletal element. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent... to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent...encoding fluorescent proteins tagged with genes or peptides with known subcellular localization patterns. ...important to determine where your protein of interest resides. There are a variety of methods for determining...red fluorescent protein (see this fusion protein guide to find the appropriate backbone). Then, by examining...

  17. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones More Fluorescent... For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog...product of exctinction coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity...Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically optimized for...

  18. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... Synthetic Biology SynBio Guide Synthetic Biology: Assembly Standards Guide SynBio Resources SynBio Home...after the promoter, and so on. The following table provides information for the assembly standards most commonly...standards, there are additional sites which should be avoided whenever possible. Other popular cloning strategies...MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods. BioBrick...

  19. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to ...deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return...

  20. Adenovirus Plasmids

    Type
    Collection
    ...Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination...for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral...genome that can be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit...Adenovirus Plasmids Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids...generate adenovirus particles. Read our Adenoviral Guide for more information about: Adenoviral components...
Showing: 21 - 40 of 137 results