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We narrowed to 133 results for: Ide

Showing: 21 - 40 of 133 results

  1. CRISPR Guide

    Type
    Collection
    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...making CRISPR perfect for genome-wide screens. This guide will provide a basic understanding of CRISPR ...genomic target of the gRNA can be any ~20 nucleotide sequence, provided it meets two conditions: The sequence...discuss more Cas9 fusion tools later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced...Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined measure of fidelity, so sometimes...with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum rodentium ) has increased fidelity, and recognizes...some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) ...

  2. TALEN Guide

    Type
    Collection
    ... TALENs TALEN Guide Addgene's Guide to TALEN Technologies...Scientists have identified a large number of zinc fingers that recognize various nucleotide triplets. With...Guide to TALEN technology and using TALEN plasmids TALENs...disciplines are coming closer to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...

  3. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top Plant ID Plasmid Gene/Insert ...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require...require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...

  4. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...Genomic Loci Mammalian Bacteria Yeast Resources Identifying molecules associated with a genomic region of... are more straightforward and enable direct identification of molecules associated with a genomic region... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...

  5. Caltech Systemic Capsids

    Type
    Collection
    ...opens in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306...plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-...plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP... (Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-...

  6. Fluorescent Protein Guide

    Type
    Collection
    ...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's...contains thousands of fluorescent proteins. Use this guide to learn more about the many applications of fluorescent...genetically encoded fluorescent biosensors. Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit...signals, cells, or groups of cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to ...Localization Determine where your protein of interest resides by using a well-characterized fluorescent fusion...

  7. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid...Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter PI Publication Return... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...

  8. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6...Available as a Viral Vector Packaging Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1...this table based on plasmid name, capsid, and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This...based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral... virus. Instead, specific gene products can be provided by helper plasmids (e.g., pHelper) and specific...HEK293 cells) during AAV production. Read our AAV Guide for more information about: AAV components Common...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...e.g. "RCaMP", "caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen peroxide Monitoring the ...Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular hydrogen peroxide concentration...

  10. Viral Vector Guides and Plasmids

    Type
    Collection
    ...viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . Resources Addgene’s ...viral vector guides and plasmids available at Addgene and other related resources. Science... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...find viral production and transfer plasmids for a wide array of research purposes. Interested in Addgene's...permanently integrated into the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Return to top Other Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter..., Dre, etc.) or target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene...selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled...empty backbones or reporters . Experimental Considerations Recombinase Recognition Sites Each enzyme functions...

  12. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...

  14. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers of cytoskeletal element. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent... to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent...important to determine where your protein of interest resides. There are a variety of methods for determining...Labeling these structures can be quite useful for identifying the localization or possible function of a protein... (1-22 aa) import sequence and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP...

  16. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ... Synthetic Biology SynBio Guide Synthetic Biology: Assembly Standards Guide SynBio Resources SynBio Home...after the promoter, and so on. The following table provides information for the assembly standards most commonly...standards, there are additional sites which should be avoided whenever possible. Other popular cloning strategies...MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods. BioBrick...

  17. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ...proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to ...deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return...

  18. Adenovirus Plasmids

    Type
    Collection
    ...Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination...for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral...genome that can be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit...Adenovirus Plasmids Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids...generate adenovirus particles. Read our Adenoviral Guide for more information about: Adenoviral components...

  19. COVID-19 Resources

    Type
    Collection
    ...COVID-19 that may be of use to scientists. Antibodies ID Recombinant Antibody Description PI 211756 Anti-SARS-CoV...please see our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene...Coronavirus Plasmids: SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control...SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry PI Additional Addgene...detection assays Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding... or Inserts Several mammalian genes have been identified as having a key role in coronavirus infection...and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools Browse the...

  20. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...and other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable ...Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication... by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR...
Showing: 21 - 40 of 133 results