We narrowed to 58 results for: TIM

...embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, and transparency...proteins to visualize and track clones of cells over time. Examples of useful tools for studying cells that...clonal origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish...vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live...

...Explore plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene ...Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. CRISPR Sylvestre...expression cassettes. A CRISPR/Cas system designed and optimized for work in the model plant Nicotiana benthamiana...Siliques Tips for Arabidopsis Transformation Save Time with Transient Plant Leaf Transformations Engineering...

...characterized by the progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases...Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive...motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to limb weakness,...CRISPRoff-v2.1 . (Nuñez et al. Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons...

... uses an open-source algorithm to automate and optimize gRNA and HDR donor design. The current version...Link opens in a new window) Program for designing optimal gRNAs. Provides feedback on number of potential...human. Developed by the George Church Lab . CRISPR Optimal Target Finder (Link opens in a new window) Identification...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning...

...mutant to inhibit MMR PEmax — optimized PE2 enzyme containing RT optimized for human codons, additional...PE2–PE5 systems PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding...

...Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags Additional Resources The following...drops to half its maximum value. Maturation is the time for fluorescence to reach half its maximal value... Bacterial Expression Return to top Fluorescent Timers Fluorescent protein variants that change their ...their fluorescence from the blue to red over time, allowing tracking of processes such as intracellular trafficking...

...Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB...pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI...

...generated using standard methods that have been optimized for each specific virus in order to generate high...Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...production, secretion, and codon-optimized versions designed for your expression system. ...SARS-CoV materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes...Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain and...

... TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector...Sangamo)-Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled...replacing pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

...rEcoli XpS (Bacterial strain #192872) , and an optimized phosphoserine orthogonal translation system, pSerOTS-C1... C321.ΔA (Bacterial strain #68306) , with the optimized phosphoserine orthogonal translation system, SepOTSλ...Rogulina, S., & Rinehart, J. (2023). System‐wide optimization of an orthogonal translation system with enhanced...

... for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...SARS-CoV materials are available only in the US at this time. Accessory Plasmids Envelope and Packaging Plasmids...

...but are difficult to generate due to expense and time. An alternative approach is to use synthetically...include consistency between lots and the ability to optimize the antibody’s antigen binding sequence to improve...

...constructs in precise cell types and/or developmental timeframes. Here we highlight some of the plasmids and tools...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with...

...Ras can activate a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation... 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide...

...targeting : Cas13 enzymes target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS...imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID...

...a protein so that it is responsive to specific stimuli, such as light. On a broader scale, the expanded... to UAG, RF1 function removed, improved doubling time. George Church 98564 C321.Ub-UAG-sfGFP all TAG sites...

...gene of interest. These modifications persist over time and are potentially heritable in dividing cells....

...tag, TRX, or V5) Mutations Wild type (wt) Codon-optimized expression (recode) Truncated for making pseudovirus...