... two gRNAs. Browse Plasmids by Model Organism Bacteria C. elegans Drosophila Mammalian Parasites Plant... on our popular CRISPR 101 blog series, we've organized a comprehensive CRISPR resource for you to download...

...account and affiliate it with your organization. If your organization has not ordered from Addgene in the...fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific community...

...modulated by ligands. Two of the important regions of nuclear receptors are: 1) a ligand binding domain...targets the receptor to specific DNA sequences. Ligand binding triggers a conformational change in the...

...Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS) C.elegans p-azido-L-phenylalanine...pairs of synthetase and tRNA that work for one organism may not work for another. The orthogonal synthetase...p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS...

...MA, 02139 Organizing Committee Chair: Dr. Melina Fan, Co-Founder and CSO, Addgene Organizing Committee... your permission). Materials don't have to be organized (yet), we will take care of that. Note that you...

... for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan 7. Use a FRET-based...monitor structural transitions in protein self-organization. Wan et al. Cell Rep Methods. 2022 Mar 28. Follow...Plasmid Resource page. CHDI Foundation is an organization with a mission to rapidly develop therapeutics...

...Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein DR274 42250 C. elegans BsaI none S. pyogenes...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into the genome...

...collection of synthetic biology plasmids for use in C. elegans....collection of synthetic biology plasmids for use in C. elegans. Worm Plasmids Search the table by keyword or sort...

...system designed and optimized for work in C. elegans . The protocol for customizing the sgRNA expression...are descirbed in: Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur...

...protein interactions to identify novel receptor-ligand interactions that function in early vertebrate ...vertebrate development using the zebrafish as a model organism. Please see the following references for more information...

...curated collection of plasmids for cancer research, organized by signaling pathway. ...constructs. Each collection page listed below organizes the plasmids and resources by signaling pathway...

...and changes of voltage all contribute to assess ligand action/engagement. Protein production is used for...assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated knowledgebase...

...customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols...

...particular organism, and the library is only compatible with cells derived from that organism. All of our...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...

...protein interactions to identify novel receptor-ligand interactions that function in the invasion of the...Platelet Endothelium Aggregation Receptor 1 (PEAR1) Ligand. Sun Y et al., Mol Cell Proteomics. 2015 May; 14...

...cell line. Fujita T, Kitaura F, Yuno M, Suzuki Y, Sugano S, Fujii H. DNA Res. 2017 Oct 1;24(5):537-548. ...regions by enChIP-Seq. Fujita T, Yuno M, Suzuki Y, Sugano S, Fujii H. Genes Cells. 2017 Jun;22(6):506-520...

...PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...complete description about chemogenetic receptors, ligands used for chemogenetic studies, and how to use these...

...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ) Zebrafish ...

...selection is not required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally...endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally...

...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...