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We narrowed to 65 results for: gats

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Showing: 21 - 40 of 65 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed...to SAM Library AAV In vivo Plasmids In vivo interrogation of gene function in the mammalian brain using...

  2. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing... provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence... of the backbone with BbsI followed by a quick ligation of annealed and phosphorylated oligonucleotides...

  3. Resolute Plasmid Collection

    Type
    Collection
    ...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors...and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert...Collection NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning Content last reviewed: 12 August 2025...

  4. Antibody Production

    Type
    Collection
    ...for use in live cells and will interfere with conjugation. For applications such as these, the buffer can...species-specific horseradish peroxidase (HRP)-conjugated secondary antibody. The tissue labeling pattern... with the antibody and a species-specific HRP-conjugated secondary antibody. Protein expression is compared...

  5. Distribution to Industry

    Type
    Collection
    ...end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden Gate assembly... name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for...

  6. Zebrafish Plasmid Collection

    Type
    Collection
    ...Morsch Lab. A vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly...Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino...HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders ...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...treating them. Scientists are studying protein aggregation, learning about the blood brain barrier integrity...glutamine-rich sequences are prone to misfolding and aggregation and can interfere with protein-protein interactions...other large scale studies. Researchers are now investigating the role that these additional genes may play...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions, the TetR protein ...Tet-On vector for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight...promoter rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter,...

  9. Plan Your Experiment

    Type
    Collection
    ...bound to the DNA can interfere with transcription elongation. CRISPR Activation : Target the transcription...containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning strategy will...., Sousa-Luís, R., & Proudfoot, N. J. (2023). Elongation roadblocks mediated by dCas9 across human genes...

  11. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...

  12. TALEN Engineering

    Type
    Collection
    ...desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander et al., Nat Biotechnol...al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput)...

  13. Chemogenetics Plasmids

    Type
    Collection
    ...popular in neuroscience, where they can be used to interrogate brain function and examine the relationship between...coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating or inhibitory...

  14. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication...Additional Resources Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast...

  15. Institute for Protein Innovation

    Type
    Collection
    ... historically been difficult to identify and interrogate with conventional reagents. Figure 1: Axon guidance...antibodies provide researchers with powerful tools to investigate neurotransmission, synapse formation, neural ...

  16. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids to produce viral vectors...research typically do not have the ability to propagate in the same way that wild-type viruses do. For...

  17. p53 Pathway

    Type
    Collection
    ...acetylation, which disrupt its interaction with negative regulators, increase its stability and DNA binding...activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...

  18. Open Enzyme Collection

    Type
    Collection
    ... Open Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the collection and order...Plasmids Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... experimental design, biosensors can enable investigation of a signaling pathway or measurement of a biomolecule... variants with different binding affinities or negative-control constructs used to characterize the sensor's...
Showing: 21 - 40 of 65 results