...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy...complement oligo (100 μM; see step 1.4 ), 1.0 μl 10x T4 Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide...These cycling conditions allow for digestion and ligation to occur in one reaction (see step 3.2 ). Transform...using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC. This is a representative sequencing...

... - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to ...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

...which can result in unintended system induction. Investigate whether or not your serum is tetracycline-free...Lentiviral vector for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA...Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for conditional cell line generation... Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving tTA; contains...

...; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148 , pAC149 ; gateway destination: pAC90...MAGeCK). This tool can identify positively or negatively selected sgRNAs, genes, or pathways. CrispyCrunch...recombination Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal co-injection...

...which will reveal sticky ends to enable the rapid ligation of annealed and phosphorylated oligos designed...to SAM Library AAV In vivo Plasmids In vivo interrogation of gene function in the mammalian brain using...

...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander et al., Nat Biotechnol...domain and flanked by restriction sites used for ligation into longer arrays. These 28 plasmids are available...

...Brzezinski Lab CRISPR Collection The Brzezinski lab investigates gene regulation in the context of the developing... provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence... of the backbone with BbsI followed by a quick ligation of annealed and phosphorylated oligonucleotides...

...RESOLUTE Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors...and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert...

...for use in live cells and will interfere with conjugation. For applications such as these, the buffer can...species-specific horseradish peroxidase (HRP)-conjugated secondary antibody. The tissue labeling pattern... with the antibody and a species-specific HRP-conjugated secondary antibody. Protein expression is compared...

...end-joining ligation fidelity and bias data to predict high accuracy junction sets for Golden Gate assembly... name Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for...

...repression) by interfering with transcriptional elongation, RNA polymerase binding, or transcription factor...expression cassette, useful for selection or fluorescent gating of transiently transfected cells. Co-expression...promoter and sgRNA targeting GAL4 UAS promoter (negative control) 46918 pU6-sgCD71-2 Human pSico-based ...

...Morsch Lab. A vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly...Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino...HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders ...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...

...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...

...popular in neuroscience, where they can be used to interrogate brain function and examine the relationship between...coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating or inhibitory...

...primers: FW 5'–GGTGACGGTGCTGGTTTA–3' RV 5'–TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication...Publication Additional Resources Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast...

...desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander et al., Nat Biotechnol...al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput)...

...acetylation, which disrupt its interaction with negative regulators, increase its stability and DNA binding...activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...

...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest...pmTurquoise2-T2A-Venus(L68V) Brightness standard used a negative control (no FRET) with pmVenus(L68V)-mTurquoise2...

... While plasmids are typically used to encode, propagate, and manipulate genetic information, viruses are...research typically do not have the ability to propagate in the same way that naturally occurring viruses...