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We narrowed to 74 results for: lias

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Showing: 21 - 40 of 74 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...Cell. 2019 Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Precursor Cells Lentiviral Mouse/Rat Generation of oligodendroglial cells by direct lineage conversion. Nat Biotechnol...into cortical and motor neurons. Unpublished. Ward Glial Progenitor Cells GABAergic Interneurons Lentiviral...Human Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons. Cells...

  2. CRISPR Guide

    Type
    Collection
    ...strategy yields C to A base edits, whereas in mammalian cells, it produces C to G edits. Adenine transversion...recombinase with improved integration efficiencies in mammalian cells. PASTE ( P rogrammable A ddition via S ite-specific...not suitable for all cell types or experiments. Mammalian CRISPR libraries have also been created in AAV...that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available, such as those...examples of activation systems to target genes in mammalian cells include: SunTag system — co-expression of...unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang ...protocols Video protocol for genomic deletions in mammalian cell lines Viral service at Addgene Viral plasmids...

  3. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does... RNA Targeting CRISPR Plasmids: RNA Targeting Mammalian Bacteria Plant Resources Type VI CRISPR systems... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag...CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control Plasmid CLIP-tag...Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag...MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid ACP...

  5. Plan Your Experiment

    Type
    Collection
    ...focus on making single edits using CRISPR/Cas9 in mammalian cells as an example, but many of these principles...common categories of genetic manipulations used in mammalian cells and their corresponding Cas enzymes. In ...KRAB can be more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase...straightforward method to deliver a CRISPR system into mammalian cells is to directly transfect expression plasmids...Designing and executing prime editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. ...

  6. CRISPR References and Information

    Type
    Collection
    ...w303). Developed by Venetia Pliatsika and Isidore Rigoutsos. See Pliatsika and Rigoutsos (2014) (Link ...cloning pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor...Orkin and Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott...

  7. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... al. described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more...permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific... this non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also ...has CRISPR plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. ...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...nuclease system to facilitate genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ....Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9... are two sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene ...In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich...

  9. Bacterial Expression Systems

    Type
    Collection
    ...visualization inside living or fixed bacterial and mammalian cells. Addgene Blog A Guide to Selecting Fluorescent... Fluorescent Protein Guide for a selection of mammalian and bacterial FRET-related vectors and standards...Paracoccus denitrificans , Methylobacterium extorquens Julia Vorholt 84693 pMyNT-kan Acetamidase promoter Acetamide... that vary in strength and that can be used to reliably drive expression of a gene of interest in E. coli...

  10. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... available for expression in mammalian systems and Drosophila. Mammalian ID Plasmid Gene/Insert Promoter...Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  11. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian Bacteria Resources CRISPR-associated transposases... used in bacteria genome editing, though some mammalian systems have been developed. Figure 1: Overview... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...

  12. Synthetic Biology - Overview

    Type
    Collection
    ...Browse plasmids related to metabolic pathways and auxiliary components. Networks and Gene Regulation Browse...Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper...Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing...

  13. Recombinases AAV Preps

    Type
    Collection
    ...Connie Cepko 69916 pAAV.cTNT.iCre cTnT tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE EF1a ...AiP13780: pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) minBG N/A 2 Allen Institute for Brain Science...Connie Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Viral Tools 183535 pAAV-CaMKIIa-Flpo CaMKIIa none...

  14. Brain Initiative Collection

    Type
    Collection
    ...genetically encoded dopamine sensor RdLight1 in mammalian cells. Lin Tian 125708-AAV9 pAAV-hSynapsin1-RdLight1...genetically encoded dopamine sensor RdLight1 in mammalian neurons. Lin Tian 127090-PHPeB pAAV-CAG-DIO-ChR2... in AAV production vector expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1...AAV production vector under the control of the mammalian promoter (EF1a) Francois St-Pierre 179463-AAV9...

  15. Plant Plasmids and Resources

    Type
    Collection
    ...distributes diverse seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information...database of genetic and molecular biology data for A. thaliana research. OpenPlant Synthetic Biology Research...) — Database of gene expression profiles in A. thaliana based on RNA-seq analysis. Figshare Plant Illustrations...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 19 William Hahn 23851 pDONR223-TBK1 TBK1 ALS William Hahn 23875 pDONR223-ERBB4 ERBB4 ALS William Hahn... and/or parkinsonism William Hahn 23452 pDONR223-PRKR EIF2AK2 VWM disease William Hahn 23504 pDONR223-...PINK1 PINK1 Parkinson's William Hahn 23520 pDONR223-LRRK2 LRRK2 Parkinson's William Hahn 23583 pDONR223-PANK2...Hallervorden-Spatz disease William Hahn 23620 pDONR223-FXN FXN Friedreich ataxia William Hahn 23640 pDONR223-...other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial...CSF1R CSF1R Hereditary diffuse leukoencephalopathy William Hahn 23955 YFP-Parkin PRKN YFP CMV Parkinson's ...Nolan 138134 pT7NT*-Bri2 113-231 R221E ITM2B NT T7 Familial British dementia Jan Johansson 138347 lentiCRISPR...

  17. Rett Syndrome

    Type
    Collection
    ...general introduction to CRISPR technology or the mammalian CRISPR resources for a full selection of plasmids...to find plasmids based on: Expression system (mammalian or bacterial) Insert species (mouse or human) ...28544139 Laurvick et al. 2006. Rett syndrome in Australia: a review of the epidemiology. J Pediatric . 148...

  18. Retrograde AAV viral preps

    Type
    Collection
    ...service. Retrograde construct from Alla Karpova at Janelia Research Institute....pAAV-CAG-FlpO CAG Flpo expression Recombinases Janelia Viral Tools 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato... helper plasmid was generated as a part of the Janelia Research Campus viral project team in collaboration...

  19. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...pAAV-AiE2128m-minBG-SYFP2-WPRE3-BGHpA AiP1357 AiE2128m SYFP2 Endothelial cells Whole Brain 214497 pAAV-AiE2135m-minBG-...pAAV-AiE2135m-minBG-SYFP2-WPRE3-BGHpA AiP1427 AiE2135m SYFP2 Endothelial cells Whole Brain 229925 pAAV-AiE2135m-minBG-iCre...pAAV-AiE2135m-minBG-iCre(R297T)-BGHpA AiP14703 AiE2135m Cre Endothelial cells Whole Brain 208146 pAAV-AiE0395h-minBglobin-...

  20. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... CRISPR Interfere CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources Catalytically dead... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...
Showing: 21 - 40 of 74 results