...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems... Tools CRISPR Blog Posts Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both ... lab and CRISPR-X from the Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase...

...Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA...MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. ... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector...development. The TapRoot - Podcast (Link opens in a new window) - The Taproot is a podcast to understand how scientific...plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples of useful...

...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target...

...target the dCas9-repressor to your specific locus. Want more information on the wide variety of Cas enzymes...Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with...VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts...guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence...

...target the dCas9-activator to your specific locus. Want more information on the wide variety of Cas enzymes...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

...that of the Cas protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems... CRISPR requires that you have to express both a Cas protein and a target-specific gRNA in the same cell.... Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their function... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering...

...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with...sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have...to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation...

...CRISPR/Cas plasmids for labeling and visualization of genomic loci.... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...