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Showing: 81 - 108 of 108 results

  1. Microbiology Resources

    Type
    Collection
    ...and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

  2. Worm Expression Resources

    Type
    Collection
    .... elegans by combining expression of the FLP recombinase from either a heat-shock promoter or a tissue-specific...WormBook - A comprehensive, open-access collection of original, peer-reviewed chapters covering topics related...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...

  4. TALEN Guide

    Type
    Collection
    ...TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter...Bogdanove Golden Gate TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The...

  5. Lentivirus Plasmids

    Type
    Collection
    ...Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible expression...Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos 24129...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...developed. tTA was created by fusing tetR with the C-terminal domain of VP16 (virion protein 16), an essential...appropriate. If your gene of interest should be mostly inactive, and only turned on occasionally, using tetracycline...

  7. Validated gRNA Sequences

    Type
    Collection
    ... 42242 cut S. pyogenes 23360964 Joung Ebf C. intestinalis GCTGAGGGTTGGACAACAGG 59990 cut S. pyogenes 25336740...pyogenes 26178787 Winslow negative control C. intestinalis GCTTTGCTACGATCTACATT 60006 cut S. pyogenes 25336740...

  10. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI...

  12. KLF Research Plasmids

    Type
    Collection
    ...feature of the KLF family is the presence of three C-terminal Krüppel-like zinc fingers which bind to CACCC ...

  13. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable...

  16. AAV Viral Preps

    Type
    Collection
    ...Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate...

  17. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...each plasmid for more information on how it was originally used. Here are a few questions to consider when...

  18. Serotype Testing AAV

    Type
    Collection
    ...which is a sample of the purified DNA from the original plasmid that was used to make the virus. For more...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...including codon optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which is ...

  20. CRISPR Plasmids and Resources

    Type
    Collection
    ...multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications Screen...

  22. AAV for Neuronal Tracing

    Type
    Collection
    ... are naturally expressed at presynaptic nerve terminals. (4) Neurons projecting to the starter cell are...

  24. Retrovirus Plasmids

    Type
    Collection
    ...overexpression plasmid; deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation...

  25. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...prime editing. Created with BioRender.com. The original prime editing enzyme is named PE1 . PE1 and most...

  26. CRISPR Plasmids - Plants

    Type
    Collection
    ... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...

  28. Optogenetics AAV Preps

    Type
    Collection
    ...-eYFP CAG ChR2/H134R EYFP Cre dependent PHPeB Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (...
Showing: 81 - 108 of 108 results