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We narrowed to 62 results for: Mammalian expression plasmids

Showing: 41 - 60 of 62 results

  1. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service: AAV Biosensors...PancACe acetyl-CoA sensor for bacterial or mammalian expression, targeted to nucleus, cytoplasm, or mitochondria...Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr: A single-wavelength...Serotonin sensor iSeroSnFR for bacterial or mammalian expression (Constitutive or Cre-dependent) Directed...Kit from the Cohen laboratory includes 35 plasmids expressing a variety of fluorescent sensors to monitor...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Collection of plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other...

  2. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ... Backbones Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description...Industry Blues Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description...Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Fluorescent Proteins Michael Davidson...fluorescent proteins. The majority of the ORFs are of mammalian origin and include localization data found below...interest. Please visit Michael Davidson's Molecular Expressions website for additional images, tutorials, optical...

  3. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a ... Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles...

  4. CRISPR Plasmids - Yeast

    Type
    Collection
    ...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...sites. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select... Select a gRNA expression plasmid based on factors such as selectable marker or cloning method. When ...

  5. Rett Syndrome

    Type
    Collection
    ... Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the ...technology or the mammalian CRISPR resources for a full selection of plasmids expressing Cas9 and empty ...bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert species...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...

  6. Promega Plasmid Collection

    Type
    Collection
    ...Promega Plasmid Collection Promega Plasmid Collection Ordering & Availability All Plasmids Plasmid Technology...our help center . Plasmid Collection Browse, sort, or search the table below of plasmids in the collection...HaloTag", "BiT", or other terms. ID Plasmid Description Industry Plasmid Technology Overview This collection...optimized for various protein expression systems including E. coli , mammalian cells, and cell-free systems...luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid Collection Explore...luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing tags such...operational complexity. This unique collection contains plasmids developed by Promega scientists and designed to...

  7. Bacterial Expression Systems

    Type
    Collection
    ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for ...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein Visualization Protein... Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like… Genetic...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert....2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...

  9. CRISPR Guide

    Type
    Collection
    ...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...systems to target genes in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...genomic deletions in mammalian cell lines Viral service at Addgene Viral plasmids and resources at Addgene... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...

  10. CRISPR Plasmids - Parasites

    Type
    Collection
    ...CRISPR expression plasmids that have been designed for use in parasites....suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...development. This collection brings together some of the plasmids that have been used in these efforts. The content...occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries...

  11. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian systems...known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR ... al. described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more...Multiple gRNAs targeting multiple loci can be easily expressed in the same cell or organism. The ability to precisely...permanent genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific... this non-specific cleavage does not occur in mammalian cells. Purify : Epitope-tagged dCas9 can also ... in the field, and enabling access to multiple plasmids used for various platform applications. For more...

  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... any of these gRNA plasmids and review the publication associated with each plasmid for more information...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...gRNA expression vectors available from Addgene. CRISPR... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  13. Lentivirus Plasmids

    Type
    Collection
    ... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis...lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452 pLenti...

  14. CRISPR References and Information

    Type
    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ...Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available... gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...

  15. CRISPR Plasmids - Plants

    Type
    Collection
    ...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert...PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as ...variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...CRISPR expression plasmids that have been designed for use in plants. CRISPR...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...

  16. Viral Vectors

    Type
    Collection
    ...enabling long term transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...direct high but transient transgene expression. γ-Retrovirus Plasmids | Guide γ-retroviruses, commonly referred...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...

  17. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite site-specific...full catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...full catalog for plasmids expressing Flp recombinase and filter the results by expression system and vector...full catalog for plasmids expressing Dre recombinase and filter the results by expression system and vector...

  18. CRISPR Plasmids - Tagging

    Type
    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms..., which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG...well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh donor plasmid. Note that the principle...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more....bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...

  20. Antibody Guide

    Type
    Collection
    ...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies. Antibodies.... Recombinant expression validation A recombinant expression strategy is used to express the protein of...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary in... comparison of expression between different samples, by normalizing protein expression to the controls...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...
Showing: 41 - 60 of 62 results