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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 120 results for: REP

Showing: 61 - 80 of 120 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...leucine-rich repeat-containing G protein-coupled receptor 4 GPR48 LGR5 leucine-rich repeat-containing G... to the formation of a high avidity BCR through repeat cycles of expansion. Chemokines Chemokines, or ... to the formation of a high avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 ...MGC102867 SEMA5A sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ... semF SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain ...LGR1, MGC141667, MGC141668, ODG1 GAL galanin prepropeptide GALN, GLNN, GMAP, MGC40167 GALP galanin-like...GHRFR, GHRHRpsv, GRFR GHRL ghrelin/obestatin prepropeptide MTLRP, obestatin GHSR growth hormone secretagogue...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

  4. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...be used with a repair template to introduce specific edits via homology-directed repair (HDR). Want more...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

  5. Synthetic Biology - Overview

    Type
    Collection
    ... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains pre-assembled...BioBrick Box Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy...

  6. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is a “search and replace” gene...Find prime editor plasmids for (search and replace) genome editing. CRISPR...heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if the edited 3’ flap...

  7. Microbiology Resources

    Type
    Collection
    ...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus sp. Vibrio sp....plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...

  8. Lentivirus Plasmids

    Type
    Collection
    ...Biosafety Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...

  9. Antibody Guide

    Type
    Collection
    ... engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead of ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary...must also be run. Unique samples (non-biological replicates) often each require their own set of controls...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...

  10. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...

  11. Resolute Plasmid Collection

    Type
    Collection
    ...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological context...the Resolute Partnership using their RESOLUTE repository feedback form (Link opens in a new window) . ...

  12. Serotype Testing AAV

    Type
    Collection
    ...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

  13. Viral Vector Guides and Plasmids

    Type
    Collection
    ... Addgene also has a selection of in-stock viral preps available. Browse Guides and Plasmids Lentiviral...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...

  14. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa REST corepressor 2 OZ553 and OZ554 OPEN OPEN gCTCACCATCtgttggaTGCGATGGAa...

  15. Worm Expression Resources

    Type
    Collection
    ...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...Contains a set of plasmids for building homologous repair templates that incorporate a self-excising drug...

  16. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...164499 EZH2-mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP...II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...

  17. Brain Armamentarium

    Type
    Collection
    ...Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 230681... and network function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid... in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading to macroscopic...plasmid also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)...

  19. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...primate neocortex. Cell Reports , 34 (13), 108754. https://doi.org/10.1016/j.celrep.2021.108754 PMID: 33789096...Link opens in a new window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers...

  20. Depositor Collections

    Type
    Collection
    ...Plasmid Collections Depositor Collections Addgene's repository contains 162,996 plasmids, which includes plasmid...Synthetic Biology Voigt Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator ...
Showing: 61 - 80 of 120 results