... endogenous human genes Guide RNA expression plasmids for endogenous zebrafish genes...these vectors for efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol...

...Protein Plasmids & Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I...Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization and/or function of...fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based...

...(toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania...have enabled precise genetic analyses to pinpoint genes linked to parasite virulence, transmission, and ...10 guides against 8,158 predicted protein-coding genes. Do you have suggestions for other plasmids that...

...modifications to the the px458 plasmid to expand its usefulness for the lab's gene regulation and developmental...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the..., Office E, Schneider SR, Abraham J, Hensley A, Jones KL, Brzezinski JA Development. 2021 Jun 15;148(...

.... A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen....Cre-lox-regulated conditional RNA interference from transgenes. Ventura A, Meissner A, Dillon CP, McManus M,...lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic...

...a variety of pathways, which ultimately turn on genes involved in cell growth, differentiation and survival... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam K, Schreck...

...PI FusX TALEN Assembly System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD ...mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction ... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...

...Regulation More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics...modular components or take advantage of existing GFP-lines for cell-specific manipulation. Plasmids Fluorescent...

...Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized Pool...

...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect...enables robust, noiseless knockdown of bacterial genes. Molecular Systems Biology, 2018 Mar 8;14(3):e7899...

...contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled...

...Design Tools CRISPR Blog Posts We adapted the S. pyogenes CRISPR/Cas9 system for use in C. elegans . ...plasmids are described in: CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans . Waaijers S, Portegijs...

... Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi...

... lists gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that... effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence designed...

...the germline. Using an injection approach, stable lines with targeted genome alterations can be generated... donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site...

...TALENs for Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene...

...is designed to target promoters or enhancers of genes of interest. Different flavors of activators were...described in: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator...

... a ligand binding domain, which interacts with hormones or other molecules, and 2) a DNA binding domain...homeostasis, and the receptors and their downstream genes are associated with numerous disease pathways. In...

...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...

...organisms, or use our Search page to look for specific genes or applications that are not listed here. Addgene...Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. Pseudomonas ...