We narrowed to 137 results for: ica
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TypeCollection...Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI Publication Yeast ID Plasmid...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease...Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should be added...
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Synthetic Biology - Cloning and Genomic Tools
TypeCollection...Genomic Tools for library construction, genomic modification, and cloning systems. Jump to Table: Cloning... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle Vectors Search the table ... or sort by the table headings. Click on the publication link to view all other plasmids from that article...Description Vector Type Selectable Marker PI Publication Back to Top Do you have suggestions for other... -
CRISPR References and Information
TypeCollection...pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation... Target Finder (Link opens in a new window) Identification of gRNA target sequences from an input sequence...window) A collection of protocols, materials, and publications by members of the Division of Signaling and ...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ... -
Antibodies
TypeCollection...expressed from plasmids. These antibodies undergo application-specific validation and consistent quality control...and provide experimental data for recommended applications. Addgene values transparency and accessibility...you choose and use antibodies, with an expert technical support team ready to answer your questions. All...antibodies developed and validated for many research applications. Browse Antibodies Production Interested in ... storage techniques, and explain common applications. View Guide Need Some Help? Browse some of our... -
CRISPR Plasmids - RNA Targeting
TypeCollection...Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert PI Publication Plant ID Plasmid Gene...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional...Gene/Insert PI Publication Do you have suggestions for other plasmids that should be added to this list?... -
CRISPR Plasmids - gRNAs
TypeCollection...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...upstream of a 5' NGG 3' PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR...CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing...plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that... -
Trimmer Lab NeuroMab Collection
TypeCollection...] VGlut1 Rat Mouse IgG2a 128639 Anti-Versican [N351/23R] Versican Mouse Mouse IgG2a 128640 Anti-Pan-Ankyrin... Lgi1 Mouse Mouse IgG2a 177528 Anti-Brevican [N294A/10R] Brevican Rat Mouse IgG2a 177529 Anti-GluN1 [N308...ARHGAP4 Mouse Mouse IgG2a 190309 Anti-Brevican [N294A/6] Brevican Rat Mouse IgG2a 190311 Anti-NSD1 [N312...Ppp1r9a Mouse Mouse IgG2a 211585 Anti-Versican [N351/24R] Versican Rat Mouse IgG2a 211587 Anti-GluN3A/NR3A...Synaptotagmin-7 Mouse Mouse IgG2a 220429 Brevican [N294A/10R-2b] Brevican Rat Mouse IgG2a 220430 BAF53b [N332B...external) Human Mouse 206772 Brevican scFv [N294A/6] N294A/6 scFv Brevican Rat Mouse 206773 GluN3A/NR3A...extensively validated for neuroscience research applications. A substantial number of these have been cloned... -
AAV Molecular Tools
TypeCollection... Tools for Affinity Purification These AAV encode tools for affinity purification (which can be used for...tet-off transactivators and tools for affinity purification (TRAP). Viral... Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell Ablation Tetracycline Transactivators...of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato 8 Scanziani Tools for Cell... -
Genetic Code Expansion
TypeCollection...incorporate a non-canonical amino acid into the protein of interest: The non-canonical amino acid, which...They are typically expressed from a single plasmid, with multiple copies of the tRNA. Applications By making...aaRS) that has been evolved to incorporate non-canonical amino acids (ncAAs). A plasmid containing the ...the gene of interest with the modified codon (typically the amber codon) that is recognized by the cognate...plasmids have been introduced in the cells, the non-canonical amino acid can be incorporated using the existing... -
Synthetic Biology - Overview
TypeCollection...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository...biology describes the rational engineering of biological systems for novel functions. Addgene is working...Browse plasmids related to cloning and genomic modification, including shuttle, integration, reporter, and...Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids...