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Showing: 1 - 40 of 40 results

  1. CAMK2A

    Plasmid
    #38971
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2A (CAMK2A Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pX330_Camk2a sgRNA / hSpCas9

    Plasmid
    #172839
    Purpose
    Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172839-plasmid-map-sequence-id-341073

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  13. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

    Plasmid
    #133732
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.
    Depositor
    Erin Schuman
    Article
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Mutation
    Promoter
    pCamk2a and pTRE3G-Bi
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    133732-plasmid-map-sequence-id-266808

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  21. pCAG-mEGFP-CaMKIIa (T305D/T306D)

    Plasmid
    #127611
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 305/306 to Alanine
    Promoter
    pCAG
    Available since
    Jan. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    127611-plasmid-map-sequence-id-250748

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  22. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid
    #127392
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid and Threon…
    Promoter
    pCAG
    Available since
    July 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    127392-plasmid-map-sequence-id-250433

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  25. pMM403_CaMKII-promoter

    Plasmid
    #34926
    Depositor
    Mark Mayford
    Insert
    CaMKII promoter (Camk2a Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKII
    Available since
    Aug. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    34926-plasmid-map-sequence-id-122246

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  37. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

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  39. pCAG-2dV-Camui (T305D/T306D)

    Plasmid
    #220368
    Purpose
    T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    changed both Threonine 305 and 306 to aspartic ac…
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220368-plasmid-map-sequence-id-469102

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  40. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit
    #1000000014
    Purpose
    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.
    Depositor
    David Root, William Hahn
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 40 of 40 results