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Showing: 1 - 40 of 1183 results

  1. pMAH-POLY

    Plasmid
    #64915
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-
    Inserts
    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    64915-plasmid-map-sequence-id-142448

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  4. pT7CFE1_polio_3ABC

    Plasmid
    #212986
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212986-plasmid-map-sequence-id-420355

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  5. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

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  6. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

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  7. pT7CFE1_polio_3ABC_C147R

    Plasmid
    #212985
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    cysteine 147 in 3C changed to arginine
    Promoter
    T7
    Available since
    Feb. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212985-plasmid-map-sequence-id-422575

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  8. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

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  9. pT7CFE1_polio_VP12A_C109R

    Plasmid
    #212987
    Purpose
    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney VP12A C109R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Cysteine 109 from 2A mutated to arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212987-plasmid-map-sequence-id-420359

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  12. pPolI_SARS-CoV-2_NLuc

    Plasmid
    #183953
    Purpose
    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
    Depositor
    AJ te Velthuis
    Article
    Vial et al bioRxiv 2021.06.28.450211
    Insert
    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mammalian Pol I
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183953-plasmid-map-sequence-id-365335

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  16. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid
    #131232
    Purpose
    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    DNA polymerase iota (POLI Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains inactivating mutations in both ubiquitin…
    Promoter
    CMV enhancer + CMV promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131232-plasmid-map-sequence-id-267278

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  31. pJRM919_WT aa 1-419 His POLI

    Plasmid
    #201445
    Purpose
    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    DNA polymerase iota (POLI Human)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Expresses amino acids 1-449 of DNA polymerase iot…
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201445-plasmid-map-sequence-id-395334

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  33. pCMV6-AN-DDK_3KA + S580A POLI

    Plasmid
    #131241
    Purpose
    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    DNA polymerase iota (POLI Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert Pol iota se…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131241-plasmid-map-sequence-id-267654

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  36. pspCas9-POLI-ST2-com vector

    Plasmid
    #176234
    Purpose
    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace
    Depositor
    Baisong Lu
    Article
    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    176234-plasmid-map-sequence-id-348845

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  38. pOT2-poly-cis

    Plasmid
    #141177
    Purpose
    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter
    Depositor
    Guiliang Tang
    Type
    Empty backbone
    Use
    RNAi and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S promoter
    Available since
    Oct. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    141177-plasmid-map-sequence-id-299765

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Showing: 1 - 40 of 1183 results