We narrowed to 1,236 results for: poli
-
-
pMAH-POLY
Plasmid#64915PurposeEncodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertsPolyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA
TagsNoneExpressionMammalianPromoterCMV and U6/H1Available SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWT PolI
Plasmid#11721DepositorAvailable SinceAug. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_3ABC
Plasmid#212986PurposeEncodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney 3ABC
UseMammalian cell free protein expressionTagsHAPromoterT7Available SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPolI-PA-66
Plasmid#240810PurposeExpresses influenza A virus mvRNA PA-66DepositorInsertmvRNA PA-66
ExpressionMammalianAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPolI-PA-60
Plasmid#240809PurposeExpresses influenza A virus mvRNA PA-60DepositorInsertmvRNA PA-60
ExpressionMammalianPromoterPol IAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_3ABC_C147R
Plasmid#212985PurposeEncodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney 3ABC C147R
UseMammalian cell free protein expressionTagsHAMutationcysteine 147 in 3C changed to argininePromoterT7Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_VP12A
Plasmid#212988PurposeEncodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus type I Mahoney VP12A
UseMammalian cell free protein expressionTagsHA tagPromoterT7Available SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_VP12A_C109R
Plasmid#212987PurposeEncodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney VP12A C109R
UseMammalian cell free protein expressionTagsHAMutationCysteine 109 from 2A mutated to argininePromoterT7Available SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PoliIFN-γ
Plasmid#107289PurposePlasmid can be used for secretion of fish Interferon gammaDepositorInsertInterferon gamma
ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPolII-Luc
Plasmid#17223DepositorInsertPolII promoter
UseLuciferaseExpressionMammalianMutationNoneAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPolI-NP71.2_inverted
Plasmid#240808PurposeExpresses influenza A virus synthetic mvRNA NP71.2DepositorInsertmvRNA NP71.2
ExpressionMammalianPromoterPol IAvailable SinceSept. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPolI-NP71.6
Plasmid#240813PurposeExpresses influenza A virus synthetic mvRNA NP71.6DepositorInsertmvRNA NP71.6
ExpressionMammalianPromoterPol IAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPolI-NP71.1
Plasmid#240807PurposeExpresses influenza A virus synthetic mvRNA NP71.1DepositorInsertNP71.1
ExpressionMammalianAvailable SinceAug. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorExpressionMammalianPromoterMammalian Pol IAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLI
Plasmid#201440PurposeExpresses human DNA polymerase iota with an N-terminal HA tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
TagsHAExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM919_WT His POLI
Plasmid#201444PurposeLow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tagDepositorInsertDNA polymerase iota (POLI Human)
Tags6x HisExpressionBacterialMutationCoding sequence has been optimised for expression…PromoterlacIqAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLI
Plasmid#131228PurposeExpression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationCoding sequence has been codon optimised for expr…PromoterCMV enhancer + CMV promoterAvailable SinceDec. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-hPOLISc
Plasmid#60819Purposeto express human Pol iota recoded to yeast codons in yeastDepositorInsertPOLI (POLI Human)
Tagsc-mycExpressionYeastMutationoptimized to yeast codon usagePromoterGAL1Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
apoeb (apolipoprotein Eb)_L (OZ529)
Plasmid#27206DepositorInsertZinc finger array targeting apoeb (apoeb Zebrafish)
UseZebrafish targetingAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
apoeb (apolipoprotein Eb)_R (OZ530)
Plasmid#27207DepositorInsertZinc finger array targeting apoeb (apoeb Zebrafish)
UseZebrafish targetingAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-dCas9-PolI5M
Plasmid#249065PurposeExpresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.DepositorInsertdCas9-PolI5M
UseCRISPRExpressionMammalianAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
RS314-U6(PolII-Lsm)
Plasmid#200772PurposePol II expression of U6 LsmDepositorInsertGal-SNR6-II
ExpressionBacterial and YeastAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S433A POLI
Plasmid#131235PurposeMammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S497A POLI
Plasmid#131236PurposeMammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S520A POLI
Plasmid#131237PurposeMammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S580A POLI
Plasmid#131238PurposeMammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_3KA POLI
Plasmid#131239PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446ADepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains point mutations that convert lysine resi…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RLUC POLIRES FLUC
Plasmid#45642PurposeBicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.DepositorInsertRLUC - IRES - FLUC
UseLuciferaseExpressionMammalianAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-420 POLI
Plasmid#201582PurposeExpresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
TagsHAExpressionMammalianMutationExpresses amino acids 1-420 of DNA polymerase iotaAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRM919_WT aa 1-419 His POLI
Plasmid#201445PurposeLow copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLIDepositorInsertDNA polymerase iota (POLI Human)
Tags6x HisExpressionBacterialMutationExpresses amino acids 1-449 of DNA polymerase iot…Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_RKK/AAA POLI
Plasmid#131240PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530ADepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains point mutations that convert arginine re…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_3KA + S580A POLI
Plasmid#131241PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580ADepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains point mutations that convert Pol iota se…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-590 POLI
Plasmid#131233PurposeMammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only
