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Plasmid#99771PurposeExpresses S. meditearranea PAMDepositorInsertPAM
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWL502-PAM
Plasmid#174382PurposePlasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterraneiDepositorInsertPAM-spacer
UseCRISPR; Archaeal expressionTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
ALL-PAM sensor Library
Pooled Library#179219Purpose10 human and mouse sgRNAs each where for each sgRNA the tethered target site has 1 of 64 possible 3 bp PAM sequences.DepositorExpressionMammalianUseLentiviralAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p11-3’_8xN_PAM-site1 (RTW554)
Pooled Library#160132PurposePlasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11-5’_10xN_PAM-site4 (RTW574)
Pooled Library#160135PurposePlasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11-3’_8xN_PAM-site2 (RTW555)
Pooled Library#160133PurposePlasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)DepositorSpeciesSyntheticUseCRISPRAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry3-C1
Plasmid#31933DepositorInsertPAmCherry3
UseTagsExpressionMammalianMutationM18L/A58T/K69N/L84F/M137I/E146D/S148L/I165L/Q167A…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAM1
Plasmid#51401PurposeExpresses truncated version of NLL-MetRS from endogenous MetG promoterDepositorInsertMetRS (metG E coli)
UseTagsExpressionMutationL13N, Y260L, H301L; Please note that a truncated …PromoterMetGAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry2-C1
Plasmid#31932DepositorInsertPAmCherry2
UseTagsExpressionMammalianMutationM18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
PAM2
Plasmid#198741PurposepGAPDH-GFP, Geneticin resistanceDepositorInsertGFP
UseAcanthamoeba expressionTagsExpressionMutationPromoterpGAPDHAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
M3-PAmcherry1
Plasmid#114189Purposestudying clustering of M3 receptors by superresolution microscopy (PALM)DepositorInsertMuscarin acetylcholine receptor 3 (CHRM3 Human)
UseTags3xHA and PAmCherryExpressionMammalianMutationPromoterSV40Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry1-N1
Plasmid#31928DepositorInsertPAmCherry1
UseTagsExpressionMammalianMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromotercmvAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAM1
Plasmid#198740PurposepTFIID-GFP, Geneticin resistanceDepositorInsertGFP
UseAcanthamoeba expressionTagsExpressionMutationPromoterpTFIIDAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry1-C1
Plasmid#31929DepositorInsertPAmCherry1
UseTagsExpressionMammalianMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM2991
Plasmid#40248DepositorInsertOne Step Cloneing Vector for Overexpression
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM1619
Plasmid#40244DepositorInsertPpsbAI::luxCDE
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only