We narrowed to 4,267 results for: Pam

1. PAM

   Plasmid

   #99771

   Purpose

   Expresses S. meditearranea PAM

   Depositor

   Phillip Newmark

   Article

   Iyer et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   PAM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PAM

   Plasmid

   #99771

   Purpose

   Expresses S. meditearranea PAM

   Depositor

   Phillip Newmark

   Article

   Iyer et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   PAM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ALL-PAM sensor Library

   Pooled Library

   #179219

   Purpose

   10 human and mouse sgRNAs each where for each sgRNA the tethered target site has 1 of 64 possible 3 bp PAM sequences.

   Depositor

   Lukas Dow

   Article

   Sanchez-Rivera et al Nat Biotechnol. 2022 Feb 14. pii: 10.103

   Expression

   Mammalian

   Use

   Lentiviral

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

6. p11-3’_8xN_PAM-site1 (RTW554)

   Pooled Library

   #160132

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

7. p11-5’_10xN_PAM-site4 (RTW574)

   Pooled Library

   #160135

   Purpose

   Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

8. p11-3’_8xN_PAM-site2 (RTW555)

   Pooled Library

   #160133

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pAM4787

   Plasmid

   #120088

   Purpose

   Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).

   Depositor

   Susan Golden

   Article

   Chen et al Microbiology. 2016 Dec;162(12):2029-2041

   Insert

   Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAM4787

    Plasmid

    #120088

    Purpose

    Cyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAM5406

    Plasmid

    #132661

    Purpose

    RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAM5406

    Plasmid

    #132661

    Purpose

    RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAM5404

    Plasmid

    #132660

    Purpose

    RSF1010-based broad host-range plasmid with additional RK2 bom site

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAM5409

    Plasmid

    #132662

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAM5404

    Plasmid

    #132660

    Purpose

    RSF1010-based broad host-range plasmid with additional RK2 bom site

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAM5409

    Plasmid

    #132662

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJethlpAmScarletCm

    Plasmid

    #206818

    Purpose

    Template for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJethlpAmNeonGreenCm

    Plasmid

    #206814

    Purpose

    Template for hlpA-mneongreen amplification associated with chloramphenicol resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    mNeonGreen

    Expression

    Mutation

    3insG

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge