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We narrowed to 611 results for: prime editing

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Showing: 271 - 300 of 611 results

  1. PB-PE xCas9

    Plasmid
    #172652
    Purpose
    All-in-one prime editor piggyBac transposon (xCas9)
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172652-plasmid-map-sequence-id-346754

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  2. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

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  10. pHSG1C3

    Plasmid
    #164423
    Purpose
    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164423-plasmid-map-sequence-id-338872

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  14. PB-PE NG

    Plasmid
    #174554
    Purpose
    All-in-one prime editor piggyBac transposon, NG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R
    Promoter
    CAG
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    174554-plasmid-map-sequence-id-354966

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  15. pU6:3-tevopreq1

    Plasmid
    #190909
    Purpose
    Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Insert
    Empty Backbone
    Use
    CRISPR
    Expression
    Insect
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190909-plasmid-map-sequence-id-373031

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  17. PB-PE SpG

    Plasmid
    #179081
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  22. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid
    #187459
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187459-plasmid-map-sequence-id-367469

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  23. ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1

    Plasmid
    #187456
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187456-plasmid-map-sequence-id-367464

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  24. ATP1A1_G6_T804N_Dual_pegRNA

    Plasmid
    #173200
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173200-plasmid-map-sequence-id-339515

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  29. pCMV-PE2-pegRNA-ST2-com-vector

    Plasmid
    #136272
    Purpose
    Expresses PE2 and ST2-com modified pegRNA cassette
    Depositor
    Baisong Lu
    Insert
    PE2, pegRNA scaffold
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    136272-plasmid-map-sequence-id-273277

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Showing: 271 - 300 of 611 results