We narrowed to 4,937 results for: Pet2

1. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Promoter

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28-2H12

   Plasmid

   #221186

   Purpose

   His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-RILP

   Plasmid

   #74600

   Purpose

   used for expression in E. coli and purification

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab-interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28b-NM23-H1

   Plasmid

   #157987

   Purpose

   Expresses NM23-H1 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Han et al Bulletin of the Kor Chem Soc 2010; 31(5)

   Insert

   NM23-H1 (NME1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GSK3beta/pET29b

   Plasmid

   #16315

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   GSK3beta (gsk3b.L Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET22HT-PP7delFG

   Plasmid

   #31863

   Depositor

   Robert Singer

   Article

   Chao et al Nat Struct Mol Biol. 2008 Jan;15(1):103-

   Insert

   PP7delFG Coat Protein
   

   Use

   Tags

   His6-tag and TEV site

   Expression

   Mutation

   deleted FG loop

   Promoter

   Available since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28b-FOXP2_P6

   Plasmid

   #122649

   Purpose

   Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

   Depositor

   Hendrik Dietz

   Article

   Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

   Insert

   Homo sapiens forkhead box P2 (FOXP2 Human)
   

   Use

   Tags

   His-tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-cMSPdH5

   Plasmid

   #199771

   Purpose

   Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH5
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET23a-HsRPS27a

    Plasmid

    #69561

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET28a(+)/FaGH16A

    Plasmid

    #100910

    Purpose

    Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FaGH16A
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-hDcp2

    Plasmid

    #72214

    Purpose

    bacterial expression of His-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET28_6xHis-PARP1delCAT

    Plasmid

    #173942

    Purpose

    Bacterial expression of PARP1 lacking CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delCAT (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 661-1014

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET29b-clbO

    Plasmid

    #114153

    Purpose

    Expresses ClbO

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbO
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET26b-Granzyme A (S-A)

    Plasmid

    #8825

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A inactive (GZMA Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    changed Serine 184 to Alanine

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pET28_6xHis-PARP1delWGR

    Plasmid

    #173940

    Purpose

    Bacterial expression of PARP1 lacking WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28GST-LIC

    Plasmid

    #26101

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6XHis, GST, and Thrombin CLeavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET26b_AT118-L-V5-His

    Plasmid

    #222848

    Purpose

    E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-clbK

    Plasmid

    #114152

    Purpose

    Expresses ClbK

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbK
    

    Use

    Tags

    6xhis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET29b-clbI

    Plasmid

    #114151

    Purpose

    Expresses ClbI

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET29b-clbF

    Plasmid

    #114148

    Purpose

    Expresses ClbF-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-clbC

    Plasmid

    #114145

    Purpose

    Expresses ClbC-nHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbC
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET29b-clbE

    Plasmid

    #114147

    Purpose

    Expresses ClbE-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbE
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET29b-clbD

    Plasmid

    #114146

    Purpose

    Expresses ClbD-cHis

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    ClbD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET29b-clbG

    Plasmid

    #114150

    Purpose

    Expresses ClbG

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET29b-clbBpks

    Plasmid

    #114154

    Purpose

    Expresses the PKS module of ClbB

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbB
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Only contains the PKS module of ClbB

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21-His6dNLP

    Plasmid

    #105498

    Purpose

    Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

    Depositor

    James Kadonaga

    Article

    Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

    Insert

    His6dNLP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-Cut-P(11)4

    Plasmid

    #185785

    Purpose

    Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    Cut-P(11)4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET21a.Tat(48-57)-I-SceI

    Plasmid

    #165041

    Purpose

    Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    I-SceI
    

    Use

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-clbH-A1

    Plasmid

    #114149

    Purpose

    Expresses ClbH-A1

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbH-A1
    

    Use

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Contains only the A1 domain of ClbH

    Promoter

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28-Rsn2

    Plasmid

    #61686

    Purpose

    bacterial expression of ranaspumin 2 as a His-tagged fusion protein

    Depositor

    Brian Smith

    Article

    Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

    Insert

    Ranaspumin-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET26b-SET

    Plasmid

    #8822

    Depositor

    Judy Lieberman

    Article

    Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

    Insert

    SET (SET Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

35. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET22b-CPDSacI

    Plasmid

    #38255

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET22-HT/spoR

    Plasmid

    #73631

    Purpose

    Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

    Depositor

    James Williamson, T. J. Kappock

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    guanylate kinase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-B4E

    Plasmid

    #162067

    Purpose

    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

    Depositor

    Karen Polizzi

    Article

    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1

    Insert

    ampR-eIF4E fusion protein
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    eIF4E incorporating the K119A mutation, which is …

    Promoter

    T7

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21c-eIF4A

    Plasmid

    #37230

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF1 (TIF1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pET21-Drp1

    Plasmid

    #72927

    Purpose

    Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    6xHis-tag and PreScission Protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge