We narrowed to 9,439 results for: Plat

1. PB_TRE_sg61-PDGFR_EF1a_eGFP

   Plasmid

   #186310

   Purpose

   Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg61-PDGFR
   eGFP
   

   Use

   Tags

   His, myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-Z29

   Plasmid

   #120072

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Strep-II
   

   Use

   Pcr template

   Tags

   Strep-II

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MXS_CMV::ZeoR-bGHpA

   Plasmid

   #62441

   Purpose

   MXS_chaining vector with CMV::ZeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Zeocin with CMV Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC-AdEvolve-DEST

   Plasmid

   #131692

   Purpose

   A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-CMV-IRESWT-Gbetagamma ONE-GO

   Plasmid

   #204339

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Gbetagamma ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. IVT-nCas9(H840A)

   Plasmid

   #207455

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   H840A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBC-MT2T3

   Plasmid

   #50594

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, TaU3t plus, U6-26p, T3
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. STAGR_gRNAScaffold_hU6

   Plasmid

   #102840

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-hSyn-Galpha-i1 ONE-GO

   Plasmid

   #204333

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-i1 ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pMaCTag-P34

    Plasmid

    #120030

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P05

    Plasmid

    #120016

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIVT-dCas9-EGFP

    Plasmid

    #174442

    Purpose

    in vitro transcription template for dCas9-EGFP

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T7 SP6

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAT11

    Plasmid

    #204290

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMaCTag-P28

    Plasmid

    #120039

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xHA
    

    Use

    Pcr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221-TCF7

    Plasmid

    #79498

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    TCF7 (TCF7 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMaCTag-Z05

    Plasmid

    #120048

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC025

    Plasmid

    #227597

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-hSyn-Galpha-oA ONE-GO

    Plasmid

    #204335

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-oA ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAC026

    Plasmid

    #227598

    Purpose

    Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

    Depositor

    Brittany Adamson

    Article

    Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122089

    Purpose

    U6 driven SpCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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