We narrowed to 3,403 results for: aaas

1. pBP-HexHis

   Plasmid

   #72983

   Purpose

   Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Polyhistidine (HHHHHH) N-terminal tag
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBP-Strep(II)

   Plasmid

   #72984

   Purpose

   Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Strep(II) N-terminal tag
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFusB3-1

   Plasmid

   #63486

   Purpose

   Encodes RVD positions 13-15 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NI NI
   

   Use

   TALEN

   Mutation

   none

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pFusX4-1

   Plasmid

   #63410

   Purpose

   Encodes RVD positions 10-12 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NI NI
   

   Use

   TALEN

   Mutation

   none

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pFusX3-1

   Plasmid

   #63347

   Purpose

   Encodes RVD positions 7-9 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NI NI
   

   Use

   TALEN

   Mutation

   none

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pFusX2-1

   Plasmid

   #63283

   Purpose

   Encodes RVD positions 4-6 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NI NI
   

   Use

   TALEN

   Mutation

   none

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pFusX1-1

   Plasmid

   #63219

   Purpose

   Encodes RVD positions 1-3 in TALEN assembly

   Depositor

   Stephen Ekker

   Article

   Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

   Insert

   NI NI NI
   

   Use

   TALEN

   Mutation

   none

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLL3.7 Alk.2 shRNA

   Plasmid

   #59297

   Purpose

   shRNA to mouse Alk

   Depositor

   Ulrike Heberlein

   Article

   Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

   Insert

   Alk.2 shRNA
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. GLRX3

   Plasmid

   #39036

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GLRX3 (GLRX3 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   A PCR artifact adds a C-terminal sequence includi…

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pSUPER.retro.puro-THAP11-E

    Plasmid

    #36995

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 shRNA (THAP11 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

11. plentiCRISPR-sgGOT1

    Plasmid

    #72874

    Purpose

    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgGOT1
    

    Use

    Lentiviral

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

13. CROP_C9_Puro_HDAC3

    Plasmid

    #183299

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    HDAC3
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGreenPuro-shRNA-Stx3S-C4

    Plasmid

    #99742

    Purpose

    3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    shRNA against Stx3S
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCgsgRNA_crtYf

    Plasmid

    #153338

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    crRNA targeting crtYf
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. RfxCas13d-backbone

    Plasmid

    #165078

    Purpose

    Expression of RfxCas13d sgRNAs from a U6 promoter

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    Scaffold gRNA
    

    Expression

    Mammalian

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CROP_C9_Puro_PDGFRB

    Plasmid

    #183314

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    PDGFRB
    

    Use

    Lentiviral

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-PTEN-shRNA-1320

    Plasmid

    #25638

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-1320 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSF4 CMV intron renilla TRICK CTE polyA

    Plasmid

    #84443

    Purpose

    TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    Renill-TRICK
    

    Use

    Luciferase; Frt

    Expression

    Mammalian

    Promoter

    Tet-CMV

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD428

    Plasmid

    #202081

    Purpose

    Cas9/sgRNA plasmid targeting the C-terminus of Myh9

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    

    Use

    CRISPR

    Promoter

    EF1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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