We narrowed to 10,167 results for: otos

1. Kif1A(1-489)-DHFR-myc

   Plasmid

   #117833

   Purpose

   Constitutively active Kinesin-3 motor tagged with DHFR and myc

   Depositor

   Thomas Schwarz

   Article

   Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

   Insert

   Kif1A (1-489) (Kif1a Mouse)
   

   Use

   Tags

   DHFR and myc

   Expression

   Mutation

   only aa 1-489 (motor domain)

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102891

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiV_Neo_SIK3_CR_T221A

   Plasmid

   #108107

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation),…

   Promoter

   EFS promoter

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGM59

   Plasmid

   #74785

   Purpose

   Expresses 3XFLAG::GR LBD::DAF-16A from the hsp16.48 promoter

   Depositor

   Jordan Ward, Keith Yamamoto

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Insert

   3X FLAG::GR LBD::DAF-16A (daf-16, NR3C1 Nematode, Synthetic, Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp16.48

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPC-Myc-TRFcT

   Plasmid

   #44573

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF2 where TRFH domain (aa42-241) has been replac…

   Promoter

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

6. AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

   Plasmid

   #173861

   Purpose

   Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

   Depositor

   Takao Nakata

   Article

   Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

   Insert

   AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiV_Neo_SIK3_CR_T142Q

   Plasmid

   #108109

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation),…

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-Myc-TRF1iDDR

   Plasmid

   #44576

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF1 containing the iDDR domain of TRF2 (aa407-43…

   Promoter

   Available Since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pBabe-Myc-TRFcH

   Plasmid

   #44574

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF2 Hinge domain (aa245-443) replaced by that of…

   Promoter

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pMALp2x-SCO5461(E164D)(43-204)

    Plasmid

    #42508

    Depositor

    Koji Okamoto

    Article

    Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

    Insert

    guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
    

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-42 (nucleotide 1-126); chan…

    Promoter

    tac

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pBabe-Myc-TRFcM

    Plasmid

    #44575

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    chimeric TRF (TERF2, TERF1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    TRF2 DNA-binding domain (aa 444-500) is replaced …

    Promoter

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-RhoA*

    Plasmid

    #198716

    Purpose

    Expresses gRNA resistant RhoA under the CAG promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    It has mutations that is resistant to the RhoA gR…

    Promoter

    CAG

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti hSyn AldoC

    Plasmid

    #220917

    Purpose

    Neuronal specific expression of AldoC

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    AldoC (ALDOC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti hSyn HK1

    Plasmid

    #220915

    Purpose

    Neuronal specific expression of HK1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    HK1 (HK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-Xpress-GBF1(E794K)

    Plasmid

    #206418

    Purpose

    Mammalian expression of hGBF1(E794K)

    Depositor

    Kazuhisa Nakayama

    Article

    Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

    Insert

    GBF1 (GBF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E794K

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGG198

    Plasmid

    #165605

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Secondary protospacer ('CGGCG' PAM) ins…

    Promoter

    lac

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA

    Plasmid

    #110654

    Purpose

    Lentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5Flag2-bglpA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA

    Plasmid

    #110656

    Purpose

    Lentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    mClover-AID-P2A-CD5cmyc2-bglpA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LentiV_Neo_LKB1_CR

    Plasmid

    #108112

    Purpose

    Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    LKB1 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change cytosine 333 to alanine (silent mutation)

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. opto-E-cad GFP

    Plasmid

    #203327

    Purpose

    Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

    Depositor

    Seraphine Wegner

    Article

    Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

    Insert

    Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Addition of Aslov2 domain at T133 of human E-cadh…

    Promoter

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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