We narrowed to 150,436 results for: ins

1. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48

   Plasmid

   #111747

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q48 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51

   Plasmid

   #111734

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q51 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q66

   Plasmid

   #111750

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q66 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73

   Plasmid

   #111724

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q73 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73

   Plasmid

   #111728

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q73 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79

   Plasmid

   #111725

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q79 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PA-DAD-dark

   Plasmid

   #53096

   Purpose

   Expresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation

   Depositor

   Klaus Hahn, Ronen Zaidel-Bar

   Article

   Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

   Insert

   LOV(dark)-6aa-DAD
   

   Tags

   6x His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   L47F and C49S in Venus; C450M mutation in LOV2

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52

   Plasmid

   #111748

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q52 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24

   Plasmid

   #111742

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q24 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25

    Plasmid

    #111743

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q25 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mRFP-FKBP-INPP5E(D556A)

    Plasmid

    #183678

    Purpose

    Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    mRFP-FKBP12(1-108)

    Expression

    Mammalian

    Mutation

    C641A, D556A

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85

    Plasmid

    #111736

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q85 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe-kappa-HA-dL5-myc-ADRB2

    Plasmid

    #101253

    Purpose

    Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)

    Depositor

    Marcel Bruchez

    Article

    Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871.

    Insert

    kappa-HA-dL5-myc-ADRB2 (ADRB2 Budding Yeast, Human)
    

    Use

    Retroviral

    Tags

    FAP-dL5 and HA epitope

    Expression

    Mammalian

    Promoter

    MMLV LTR

    Available Since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. BRI1 BRI1_pECIA2

    Plasmid

    #115086

    Purpose

    Bait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT4G39400 (BRI1 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI AscI MR1 res

    Plasmid

    #214752

    Purpose

    expresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cells

    Depositor

    David Lewinsohn

    Article

    Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

    Insert

    MHC class I-related protein 1 (MR1 Human)
    

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    silent mutations to prevent editing by CRISPR/Cas…

    Promoter

    CMV

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV6-MPP8-W80A-10xHis

    Plasmid

    #194179

    Purpose

    Expresses MPP8-W80A_10xHis

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

    Insert

    MPHOSPH8 (MPHOSPH8 Human)
    

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    MPP8-W80A inside chromo domain

    Promoter

    CMV

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-CMV GFP-LC3B Q116P G120

    Plasmid

    #129290

    Purpose

    Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFBD-BirA_CDC20:161-P477

    Plasmid

    #210888

    Purpose

    Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    CDC20:S161-P477 (CDC20 Human)
    

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available Since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFBOH-LIC_DDB1:1-1140_delta:396-689

    Plasmid

    #210897

    Purpose

    Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
    

    Expression

    Insect

    Mutation

    loop deletion amino acids I396 to D689

    Promoter

    Polyhedrin

    Available Since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFBOH-avi-TEV-LIC_DCAF12:1-453

    Plasmid

    #210940

    Purpose

    Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF12:M1-S453 (DCAF12 Human)
    

    Expression

    Insect

    Mutation

    R131Q natural variant (VAR_035322)

    Promoter

    Polyhedrin

    Available Since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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