We narrowed to 120,498 results for: CIL

1. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

   Plasmid

   #110300

   Purpose

   Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

   Plasmid

   #123611

   Purpose

   CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   nCas9-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

   Plasmid

   #99746

   Purpose

   Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

   Insert

   Stx3 (STX3 Human)
   

   Tags

   myc-myc-his

   Expression

   Mammalian

   Mutation

   Made six (6) silent mutations to confer resistanc…

   Promoter

   CMV/TO

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GUCY2C gRNA (BRDN0001144864)

   Plasmid

   #77563

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human GUCY2C

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   GUCY2C (GUCY2C Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-Flag-SPRTN-Y117C

   Plasmid

   #110216

   Purpose

   Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y117C, P296L (see depositor comments below)

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEAHISPApqsE

   Plasmid

   #97019

   Purpose

   Expressing Pseudomonas aeruginosa pqsE protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Quinolone signal response protein
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAHISPAFabH

   Plasmid

   #97018

   Purpose

   Expressing Pseudomonas aeruginosa FabH protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   3-Oxoacyl-[acyl-carrier-protein] synthase III
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GUCY2C gRNA (BRDN0001148670)

   Plasmid

   #77564

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human GUCY2C

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   GUCY2C (GUCY2C Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GUCY2C gRNA (BRDN0001146365)

   Plasmid

   #77565

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human GUCY2C

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   GUCY2C (GUCY2C Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. GUCY2C gRNA (BRDN0001144918)

    Plasmid

    #77566

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GUCY2C

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GUCY2C (GUCY2C Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-FLAG-CHD5-Y619E-FRAG

    Plasmid

    #68871

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa, Y619E

    Promoter

    CMV

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBOB-HA-Gnas

    Plasmid

    #245994

    Purpose

    Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gnas (Gnas Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG-PM-NGN3-linker-3xFLAG-Dendra2-HA

    Plasmid

    #234888

    Purpose

    Expresses human phosphomutant-NGN3 in mammalian cells directly fused to Dendra2 fluorescent protin plus FLAG and HA tags

    Depositor

    Nancy Papalopulu

    Article

    Miller et al Dev Cell. 2025 Sep 22;60(18):2518-2532.e

    Insert

    Phosphomutant-NGN3-3xFLAG-Dendra2-HA (NEUROG3 Human)
    

    Tags

    3xFLAG, Dendra2 and HA

    Expression

    Mammalian

    Mutation

    Changed Serines 161, 165, 174, 183 and 191 to Ala…

    Promoter

    CAG

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDual

    Plasmid

    #207883

    Purpose

    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

    Depositor

    David Edgell

    Article

    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

    Inserts

    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Leucine 212 changed to proline, leucine 434 in th…

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

    Plasmid

    #208399

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid

    #208398

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    E2-Crimson

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

    Plasmid

    #208400

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDR111_mannose_Stat1Klf6

    Plasmid

    #188400

    Purpose

    Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Inserts

    Stat-1 (Stat1 Mouse, Escherichia coli)
    Klf6 (Klf6 Mouse)
    

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Promoter

    D-mannose

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

    Plasmid

    #178854

    Purpose

    Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
    

    Expression

    Mammalian

    Mutation

    Grb2: Y160E

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

    Plasmid

    #172105

    Purpose

    Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)-Gαs (GNAS Human)
    

    Expression

    Mammalian

    Mutation

    Gαs: C3S

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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