We narrowed to 7,210 results for: CIT

1. pRS416 Gal-RNQ1 L94A-YFP

   Plasmid

   #18692

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 L94A (RNQ1 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Leucine 94 mutated to Alanine (L94A)

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

2. Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

   Plasmid

   #15602

   Depositor

   Susan Lindquist

   Article

   Tessier et al Nature. 2007 May 31. 447(7144):556-61.

   Insert

   Sup35 NM (SUP35 Budding Yeast, Candida albicans)
   

   Use

   Tags

   7xHis and Cysteine

   Expression

   Bacterial

   Mutation

   4 G/A. Four glycines mutated to alanines. The pos…

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. FH95pUP95S73DGW (F3)

   Plasmid

   #74040

   Purpose

   lentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion

   Depositor

   Bernardo Sabatini, Weifeng Xu, Robert Malenka

   Article

   Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

   Insert

   Psd95
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   S73D

   Promoter

   H1

   Available since

   Availability

   Academic Institutions and Nonprofits only

4. Human Transporter Knockout library

   Pooled Library

   #213695

   Purpose

   Next generation transporter-focused CRISPR/Cas9 library targeting transmembrane transporters to test the impact of transporters on biological processes.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Ferdigg et al Life Sci Alliance. 2024 Nov 22;8(2):e202

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

5. SPECS library

   Pooled Library

   #127842

   Purpose

   For performing high-throughput cell state-specific synthetic promoter screening

   Depositor

   Timothy Lu

   Article

   Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

   Species

   Synthetic

   Use

   Lentiviral

   Available since

   Aug. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

6. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

   Plasmid

   #170255

   Purpose

   Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   SV40 Large T Antigen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-iCas9-neo

   Plasmid

   #85400

   Purpose

   Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Insert

   Flag-iCas9-P2A-GFP
   

   Use

   Lentiviral

   Tags

   Flag-iCas9-P2A-GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuet TDP43 WT

   Plasmid

   #27462

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. plenti hSyn PKM1

   Plasmid

   #220920

   Purpose

   Neuronal specific expression of PKM1

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PKM1 (PKM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-HsSENP1

    Plasmid

    #71465

    Purpose

    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. iTol2Amp-γ-crystallin:RFP

    Plasmid

    #108455

    Purpose

    This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    Tol2-Amp-y-cryst-mCherry
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBa.Halo-LDLR

    Plasmid

    #162717

    Purpose

    Mammalian expression of human LDLR

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    LDLR (LDLR Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRIPZ shNS

    Plasmid

    #127696

    Purpose

    Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shNS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-HsSENP2

    Plasmid

    #71466

    Purpose

    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only