Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,186 results for: egf

Clear keywords
Showing: 2041 - 2060 of 15186 results

  3. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Publication
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    132393-plasmid-map-sequence-id-341619

    Enlarge

  7. pAAV-hSyn-Flex-Axon-EGFP

    Plasmid
    #135429
    Purpose
    Can be used to drive axon-enriched expression of EGFP in the presence of Cre recombinase.
    Depositor
    Rylan Larsen
    Has service
    AAV1
    Insert
    axon-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GAP43 palmitoylation domain
    Expression
    Mammalian
    Promoter
    hSyn1
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135429-plasmid-map-sequence-id-271167

    Enlarge

  9. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Publication
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

    Enlarge

  11. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid
    #188902
    Purpose
    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9-KRAB(Kox1)-MeCP2
    Use
    Lentiviral
    Tags
    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…
    Promoter
    SFFV
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188902-plasmid-map-sequence-id-372712

    Enlarge

  12. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

    Enlarge

  13. pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid
    #92140
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID[71-114]-eGFP
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    92140-plasmid-map-sequence-id-472385

    Enlarge

  15. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    109428-plasmid-map-sequence-id-212894

    Enlarge

  18. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Publication
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

    Enlarge

  20. EGF-A-SpyTag

    Plasmid
    #246944
    Purpose
    Expresses multifunctional construct for YES-gated release of Epidermal Growth Factor (EGF) from materials in response to sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Publication
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246944-plasmid-map-sequence-id-489549

    Enlarge

Showing: 2041 - 2060 of 15186 results