We narrowed to 23,428 results for: CRISPR

1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHSE401

   Plasmid

   #62201

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiSAMv2

   Plasmid

   #75112

   Purpose

   lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHUE411

   Plasmid

   #62203

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   OsU3 and Ubi

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgRNA

   Plasmid

   #124844

   Purpose

   Vector for Cre-dependent expression of SaCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SaCas9, gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   NLS and NLS-3xHA

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPlacPbuCas13b-gRNA-T-deGFP

   Plasmid

   #184840

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 and lac promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPgiCas13b-gRNA-1

    Plasmid

    #184834

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC50

    Plasmid

    #104824

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.08g081600, Glyma.05g126600
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-Tet1-CD

    Plasmid

    #83340

    Purpose

    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    U6, CBH

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-NLS-AcrIIA4

    Plasmid

    #113037

    Purpose

    Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SV40 NLS-AcrIIA4
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJYS3_ΔcrtYf

    Plasmid

    #85542

    Purpose

    Constitutive transcription of FnCpf1 and crRNA of crtYf

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ173

    Plasmid

    #99907

    Purpose

    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Insert

    pco-dCas9-VP64-T2A-MS2-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBUE411

    Plasmid

    #62200

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    OsU3 and Ubi

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS1yl

    Plasmid

    #73226

    Purpose

    Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

    Depositor

    Sheng Yang

    Article

    Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; In y. lipolytica

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-tight-DDdCas9GFP-IRES-Neo

    Plasmid

    #102907

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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