We narrowed to 3,989 results for: FAS

1. FRB_nSbMVp

   Plasmid

   #118969

   Purpose

   Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nSbMVp in fusion with FRB
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET41a iGluh

   Plasmid

   #119830

   Purpose

   Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluh
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25A

   Promoter

   T7

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. cycLuc_PPVs

   Plasmid

   #119208

   Purpose

   Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   cyclic luciferase with PPVp cleavage site
   

   Use

   Luciferase

   Tags

   AU1 tag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP_cSbMVp

   Plasmid

   #118968

   Purpose

   Expresses C-terminus of soybean mosaic virus protease (SbMVp) fused to the FK-506 binding protein (FKBP)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cSbMVp in fusion with FKBP
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW-RapACR_T111C-EYFP

   Plasmid

   #119195

   Purpose

   Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   changed threonine 111 to cysteine

   Promoter

   Ubiquitin

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-ZZ-TEV-SNAPf-BICD2N(1-400)

   Plasmid

   #111857

   Purpose

   N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

   Depositor

   Andrew Carter

   Article

   Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

   Insert

   BICD2N(1-400)
   

   Use

   Tags

   His-tag, SNAPf-tag, TEV site, and ZZ

   Expression

   Insect

   Mutation

   Optimised for Sf9 expression

   Promoter

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ptdEos-CLC

   Plasmid

   #38009

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Inserts

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   Eos FP, tandem dimer
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP_cPPVp

   Plasmid

   #118967

   Purpose

   Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cPPVp in fusion with FKBP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRB_nPPVp

   Plasmid

   #118966

   Purpose

   Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nPPVp in fusion with FRB
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. dsRedm-Rab21

    Plasmid

    #83425

    Purpose

    Full-length mouse Rab21 (wildtype)

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al Dev Cell. 2008 Sep;15(3):371-85. doi: 10

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    dsRed-monomer

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-CMV-NES-fRGECO1

    Plasmid

    #125247

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSV human p55 alpha (HA tag)

    Plasmid

    #11498

    Depositor

    Ronald Kahn

    Article

    Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

    Insert

    p55 alpha (PIK3R1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

13. cycLuc_SuMMVs

    Plasmid

    #119210

    Purpose

    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with SuMMVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHCas9-Nours

    Plasmid

    #107733

    Purpose

    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Human optimized

    Promoter

    TEF1

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI-syn-fRCaMP1

    Plasmid

    #125245

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W44Y

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJ414-PCY1

    Plasmid

    #98150

    Purpose

    Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGP-CMV-NEW-fRGECO2

    Plasmid

    #125248

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cycLuc_SbMVs

    Plasmid

    #119209

    Purpose

    Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with SbMVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSR-siKlf5

    Plasmid

    #41740

    Depositor

    Jonathan Katz

    Article

    Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

    Insert

    Klf5 (Klf5 Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pNB784

    Plasmid

    #60163

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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